Liquid chromatographic determination of fluoxetine

A simple, accurate and sensitive high pressure liquid chromatographic technique is described for the determination of fluoxetine in the capsule dosage form, human plasma and in biological fluid. Analysis is performed with a reversed phase-C18 column with ultraviolet detection at 228 nm. The isocrati...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of pharmaceutical and biomedical analysis 2002-10, Vol.30 (3), p.561-571
Hauptverfasser: El-dawy, Mohamed A, Mabrouk, Mokhtar M, El-Barbary, Fawzy A
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A simple, accurate and sensitive high pressure liquid chromatographic technique is described for the determination of fluoxetine in the capsule dosage form, human plasma and in biological fluid. Analysis is performed with a reversed phase-C18 column with ultraviolet detection at 228 nm. The isocratic mobile phase (1.5 ml/min.) consists of acetonitrile and triethylamine buffer (48+52, V/V). A linear calibration model (correlation coefficient 0.99863) was developed using pyridoxine as internal standard. The retention times were 2.10 and 3.20 min for pyridoxine and fluoxetine, respectively. The method was applied for the quantitation of fluoxetine in spiked human plasma samples. The detection limit is 5 μg/l and the absorbance varies with fluoxetine concentrations in the range (10–300) μg/l. The mean % recovery±S.D. was found to be 97.99%±2.39. The proposed method was applied successfully for monitoring of fluoxetine in human plasma after single dose administration of one prozac® capsule.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(02)00312-6