Quantitative Assessment of the SR Ca2+ Leak-Load Relationship

ABSTRACT—Increased diastolic SR Ca leak (Jleak) could depress contractility in heart failure, but there are conflicting reports regarding the Jleak magnitude even in normal, intact myocytes. We have developed a novel approach to measure SR Ca leak in intact, isolated ventricular myocytes. After stim...

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Veröffentlicht in:	Circulation research 2002-10, Vol.91 (7), p.594-600
Hauptverfasser:	Shannon, Thomas R, Ginsburg, Kenneth S, Bers, Donald M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Calcium - analysis Calcium - metabolism Calcium Channels - metabolism Cells, Cultured Diastole Fundamental and applied biological sciences. Psychology Heart Heart - drug effects Heart - physiology Heart Failure - etiology Ion Transport Kinetics Models, Cardiovascular Myocardial Contraction Myocardium - chemistry Myocardium - metabolism Rabbits Sarcoplasmic Reticulum - metabolism Space life sciences Tetracaine - pharmacology Vertebrates: cardiovascular system
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Zusammenfassung:	ABSTRACT—Increased diastolic SR Ca leak (Jleak) could depress contractility in heart failure, but there are conflicting reports regarding the Jleak magnitude even in normal, intact myocytes. We have developed a novel approach to measure SR Ca leak in intact, isolated ventricular myocytes. After stimulation, myocytes were exposed to 0 Na, 0 Ca solution ±1 mmol/L tetracaine (to block resting leak). Total cell [Ca] does not change under these conditions with Na-Ca exchange inhibited. Resting [Ca]i declined 25% after tetracaine addition (126±6 versus 94±6 nmol/L;P
ISSN:	0009-7330 1524-4571
DOI:	10.1161/01.RES.0000036914.12686.28