Evaluation of antibiotic sensitivity of Chlamydia trachomatis using RT-PCR

The sensitivity of 11 clinical strains of Chlamydia trachomatis to azithromycin, ofloxacin, doxycycline, and erythromycin was evaluated. The minimum inhibiting concentrations of all antibiotics for 90% strains, determined by PCR with reverse transcription of omp3B gene RNA (GenBank U68443) correspon...

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Veröffentlicht in:	Bulletin of experimental biology and medicine 2002-03, Vol.133 (3), p.308-310
Hauptverfasser:	Misyurina, O Yu, Shipitsina, E V, Parfenova, T M, Lazarev, V N, Savicheva, A M, Govorun, V M
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Chlamydia trachomatis - drug effects Chlamydia trachomatis - genetics DNA Primers Drug Resistance, Microbial Molecular Sequence Data Reverse Transcriptase Polymerase Chain Reaction RNA, Bacterial - genetics RNA, Bacterial - isolation & purification
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Zusammenfassung:	The sensitivity of 11 clinical strains of Chlamydia trachomatis to azithromycin, ofloxacin, doxycycline, and erythromycin was evaluated. The minimum inhibiting concentrations of all antibiotics for 90% strains, determined by PCR with reverse transcription of omp3B gene RNA (GenBank U68443) corresponded to, and those with reverse transcription of 16S rRNA gene RNA (GenBank X54451) far surpassed the minimum bactericidal concentrations for 90% strains determined by direct immunofluorescence with monoclonal antibodies to the major outer membrane protein.
ISSN:	0007-4888 1573-8221
DOI:	10.1023/A:1015815607609