Creation of a GAL4/UAS-coupled inducible gene expression system for use in drosophila cultured cell lines

Creation of a GAL4/UAS-coupled inducible gene expression system for use in drosophila cultured cell lines

The usefulness of the GAL4/UAS system as a tool for targeted gene expression in Drosophila melanogaster has been widely recognized. By combining tissue-specific GAL4 drivers with stably integrated pUAST-based responder constructs, the GAL4/UAS system transformed gene misexpression into an even more...

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Veröffentlicht in:Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2002-09, Vol.34 (1-2), p.119-122
Hauptverfasser: Klueg, Kristin M., Alvarado, Diego, Muskavitch, Marc A.T., Duffy, Joseph B.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cell Line
DNA-Binding Proteins
Drosophila melanogaster - genetics
Enhancer Elements, Genetic
Gene Transfer Techniques
Metallothionein - genetics
Promoter Regions, Genetic
Saccharomyces cerevisiae Proteins - genetics
Transcription Factors - genetics
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Zusammenfassung:The usefulness of the GAL4/UAS system as a tool for targeted gene expression in Drosophila melanogaster has been widely recognized. By combining tissue-specific GAL4 drivers with stably integrated pUAST-based responder constructs, the GAL4/UAS system transformed gene misexpression into an even more precise and powerful tool for functional analysis.
ISSN:1526-954X
1526-968X
DOI:10.1002/gene.10148