An economical and preparative orthogonal solid phase synthesis of fluorescein and rhodamine derivatized peptides: FRET substrates for the Staphylococcus aureus sortase SrtA transpeptidase reaction

An economical and preparative-scale orthogonal solid-phase method of incorporating carboxyrhodamine and carboxyfluorescein fluorescence resonance energy transfer (FRET) probes site-specifically into synthetic peptide substrates for the S. aureus Sortase transpeptidase SrtA has been developed.

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Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2002-09 (18), p.2092-2093
Hauptverfasser:	Kruger, Ryan G, Dostal, Patrick, McCafferty, Dewey G
Format:	Artikel
Sprache:	eng
Schlagworte:	Aminoacyltransferases - chemistry Aminoacyltransferases - metabolism Bacterial Proteins Cysteine Endopeptidases Energy Transfer Fluorescein - chemistry Fluorescence Polarization Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry Isoenzymes - chemistry Isoenzymes - metabolism Molecular Probe Techniques Peptides - chemical synthesis Peptidyl Transferases - chemistry Peptidyl Transferases - metabolism Rhodamines - chemistry Staphylococcus aureus - enzymology Substrate Specificity
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Beschreibung
Zusammenfassung:	An economical and preparative-scale orthogonal solid-phase method of incorporating carboxyrhodamine and carboxyfluorescein fluorescence resonance energy transfer (FRET) probes site-specifically into synthetic peptide substrates for the S. aureus Sortase transpeptidase SrtA has been developed.
ISSN:	1359-7345
DOI:	10.1039/b206303d