Production of high purity proteins by elution of individual fractions from two-dimensional gels
Results are presented on the production of purified myocardial proteins from two‐dimensional (2‐D) gels. Proteins were fixed in a native condition using potassium acetate and then eluted into an aqueous solution. Homogeneous tropomyosin and myosin light chain fractions and a number of nonidentified...
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| Veröffentlicht in: | Electrophoresis 1991, Vol.12 (7-8), p.576-578 |
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| container_title | Electrophoresis |
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| creator | Tsvetkova, Marina N. Kovalyov, Leonid I. Laptev, Alexei V. Shishkin, Sergei S. |
| description | Results are presented on the production of purified myocardial proteins from two‐dimensional (2‐D) gels. Proteins were fixed in a native condition using potassium acetate and then eluted into an aqueous solution. Homogeneous tropomyosin and myosin light chain fractions and a number of nonidentified myocardial proteins present on 2‐D gels were obtained. |
| doi_str_mv | 10.1002/elps.1150120717 |
| format | Article |
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Psychology</subject><subject>General aspects, investigation methods</subject><subject>Humans</subject><subject>Molecular Weight</subject><subject>Muscle Proteins - analysis</subject><subject>Myocardium - chemistry</subject><subject>Myosins - analysis</subject><subject>Proteins</subject><subject>Staining and Labeling</subject><subject>Tropomyosin - analysis</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1P3DAQxS3UCraUc0-VcmlvAdsTrxP11FJgK60ookUcLceegKmTbO2ksP99TbOw6omTR36_Nx-PkHeMHjJK-RH6VTxkTFDGqWRyh8yY4Dzn8xJekRllEnJagtgjb2K8o5QWVVHskl2WWC5gRtRF6O1oBtd3Wd9kt-7mNluNwQ3rbBX6AV0Xs3qdoR-fENdZ98fZUfusCfqfM6aqb7Phvs-ta7GL6S_JN-jjW_K60T7iwebdJ1enJz-PF_ny-9m348_L3ACXMheN0VQbzQsAXepGz4WlNU-HFSBqkLpGKxnaqkBBOUcLZWGgKitgZs5LhH3yceqbtv49YhxU66JB73WH_RiV5IzzgtIEHk2gCX2MARu1Cq7VYa0YVY-RqsdI1TbS5Hi_aT3WLdotP2WY9A8bXUejfQqlMy5usarkAJQl7tPE3TuP65fGqpPlxY__tsgnt4sDPjy7dfil5hKkUNfnZ6pcXMLXxZdLxeAv7qegwg</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>Tsvetkova, Marina N.</creator><creator>Kovalyov, Leonid I.</creator><creator>Laptev, Alexei V.</creator><creator>Shishkin, Sergei S.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-VCH</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1991</creationdate><title>Production of high purity proteins by elution of individual fractions from two-dimensional gels</title><author>Tsvetkova, Marina N. ; Kovalyov, Leonid I. ; Laptev, Alexei V. ; Shishkin, Sergei S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3277-5fca0aca2433a8afa65d0b2115435b37abed71ed94e5022ed384c398931c628e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Electrophoresis, Gel, Two-Dimensional - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation methods</topic><topic>Humans</topic><topic>Molecular Weight</topic><topic>Muscle Proteins - analysis</topic><topic>Myocardium - chemistry</topic><topic>Myosins - analysis</topic><topic>Proteins</topic><topic>Staining and Labeling</topic><topic>Tropomyosin - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsvetkova, Marina N.</creatorcontrib><creatorcontrib>Kovalyov, Leonid I.</creatorcontrib><creatorcontrib>Laptev, Alexei V.</creatorcontrib><creatorcontrib>Shishkin, Sergei S.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsvetkova, Marina N.</au><au>Kovalyov, Leonid I.</au><au>Laptev, Alexei V.</au><au>Shishkin, Sergei S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of high purity proteins by elution of individual fractions from two-dimensional gels</atitle><jtitle>Electrophoresis</jtitle><addtitle>ELECTROPHORESIS</addtitle><date>1991</date><risdate>1991</risdate><volume>12</volume><issue>7-8</issue><spage>576</spage><epage>578</epage><pages>576-578</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>Results are presented on the production of purified myocardial proteins from two‐dimensional (2‐D) gels. 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| fulltext | fulltext |
| identifier | ISSN: 0173-0835 |
| ispartof | Electrophoresis, 1991, Vol.12 (7-8), p.576-578 |
| issn | 0173-0835 1522-2683 |
| language | eng |
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| source | MEDLINE; Wiley Online Library |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences Blotting, Western Electrophoresis, Gel, Two-Dimensional - methods Fundamental and applied biological sciences. Psychology General aspects, investigation methods Humans Molecular Weight Muscle Proteins - analysis Myocardium - chemistry Myosins - analysis Proteins Staining and Labeling Tropomyosin - analysis |
| title | Production of high purity proteins by elution of individual fractions from two-dimensional gels |
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