Production of high purity proteins by elution of individual fractions from two-dimensional gels

Production of high purity proteins by elution of individual fractions from two-dimensional gels

Results are presented on the production of purified myocardial proteins from two‐dimensional (2‐D) gels. Proteins were fixed in a native condition using potassium acetate and then eluted into an aqueous solution. Homogeneous tropomyosin and myosin light chain fractions and a number of nonidentified...

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Veröffentlicht in:Electrophoresis 1991, Vol.12 (7-8), p.576-578
Hauptverfasser: Tsvetkova, Marina N., Kovalyov, Leonid I., Laptev, Alexei V., Shishkin, Sergei S.
Format: Artikel
Sprache:eng
Schlagworte:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Blotting, Western
Electrophoresis, Gel, Two-Dimensional - methods
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Humans
Molecular Weight
Muscle Proteins - analysis
Myocardium - chemistry
Myosins - analysis
Proteins
Staining and Labeling
Tropomyosin - analysis
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Beschreibung
Zusammenfassung:Results are presented on the production of purified myocardial proteins from two‐dimensional (2‐D) gels. Proteins were fixed in a native condition using potassium acetate and then eluted into an aqueous solution. Homogeneous tropomyosin and myosin light chain fractions and a number of nonidentified myocardial proteins present on 2‐D gels were obtained.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.1150120717