Application of self-assembly techniques in the design of biocompatible protein microarray surfaces

This review focuses on the application of novel technologies for generating biocompatible surfaces for high‐throughput screening (HTS) of proteins. Various methods of coupling and spotting proteins on self‐assembled monolayer (SAM) surfaces will be described along with the protein chip challenges pe...

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Veröffentlicht in:	Electrophoresis 2002-09, Vol.23 (18), p.3097-3105
Hauptverfasser:	Schaeferling, Michael, Schiller, Stefan, Paul, Hubert, Kruschina, Margit, Pavlickova, Petra, Meerkamp, Mark, Giammasi, Chiara, Kambhampati, Dev
Format:	Artikel
Sprache:	eng
Schlagworte:	Biocompatible Materials - chemistry Cells, Immobilized Dextrans - chemistry Electrophoresis, Gel, Two-Dimensional - instrumentation Electrophoresis, Gel, Two-Dimensional - methods Equipment Design Glass Lipid Bilayers - chemistry Materials Testing Protein Array Analysis - instrumentation Protein Array Analysis - methods Protein microarrays Proteomics Review Self-assembled monolayers Surface Properties
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Beschreibung
Zusammenfassung:	This review focuses on the application of novel technologies for generating biocompatible surfaces for high‐throughput screening (HTS) of proteins. Various methods of coupling and spotting proteins on self‐assembled monolayer (SAM) surfaces will be described along with the protein chip challenges pertaining to spot homogeneity, morphology, biocompatibility and reproducibility.
ISSN:	0173-0835 1522-2683
DOI:	10.1002/1522-2683(200209)23:18<3097::AID-ELPS3097>3.0.CO;2-G