Human fetal central nervous system organotypic cultures
Many aspects of human neurodevelopment remain enigmatic. A main reason for this is, although there have been a significant number of morphologic and biochemical studies of neural tissues derived from human embryos and fetuses, this can only provide a static picture of the events at a given gestation...
Gespeichert in:
Veröffentlicht in: | Brain research. Developmental brain research 1991-06, Vol.60 (2), p.155-160 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 160 |
---|---|
container_issue | 2 |
container_start_page | 155 |
container_title | Brain research. Developmental brain research |
container_volume | 60 |
creator | LYMAN, W. D TRICOCHE, M HATCH, W. C KRESS, Y CHIU, F.-C RASHBAUM, W. K |
description | Many aspects of human neurodevelopment remain enigmatic. A main reason for this is, although there have been a significant number of morphologic and biochemical studies of neural tissues derived from human embryos and fetuses, this can only provide a static picture of the events at a given gestational age. Also, in vitro studies that focus on cells derived from these tissues have a limitation in that different cell types in dissociated tissue culture cannot interact in a 'normal' physiologic manner thereby, perhaps, altering their housekeeping and luxury functions. The present study focused on the development of a human explant organotypic culture model that may overcome the static limitation of the first example and permit a dynamic analysis of different cell types as they interact which may satisfy the second restriction. Because there is an array of developmental markers that define different cell phenotypes, this explant model may also provide a means of analyzing, for the first time, processes undefined in the human CNS. Human fetal CNS tissue obtained from second trimester abortuses was established in culture. The tissues were maintained for up to 12 weeks during which time they developed and differentiated. Sample cultures were harvested periodically and analyzed by light- and electron microscopy in combination with immunocytochemistry. Differentiation of neurons, astrocytes, oligodendrocytes and endothelial cells was documented using morphologic and biochemical criteria. As such, this model system may allow for the analysis of processes that occur during normal human fetal neurodevelopment and in pathologic conditions. |
doi_str_mv | 10.1016/0165-3806(91)90044-J |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72099748</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72099748</sourcerecordid><originalsourceid>FETCH-LOGICAL-c382t-1fa6b8a3c08c907103af94d81b4ae1bad7b1d0b26d89a296f5156041508601953</originalsourceid><addsrcrecordid>eNpFkM1Lw0AQxfeg1Fr9DxRyENFDdCb7kd2jFL9KwYuel8lmI5V81N1E6H9vaks9DA_mvTcMP8YuEO4QUN2PI1OuQd0YvDUAQqSLIzY9rE_YaYxfAIBc44RNUBsulZyy_GVoqE0q31OdON_2YdTWh59uiEncxN43SRc-qe36zXrlEjfU_RB8PGPHFdXRn-91xj6eHt_nL-ny7fl1_rBMHddZn2JFqtDEHWhnIEfgVBlRaiwEeSyozAssochUqQ1lRlUSpQKBErQCNJLP2PXu7jp034OPvW1W0fm6ptaPL9o8A2Nyoceg2AVd6GIMvrLrsGoobCyC3TKyWxh2C8MatH-M7GKsXe7vD0Xjy__SDtDoX-19io7qKlDrVvEQE0YalRv-Cy4-cBE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72099748</pqid></control><display><type>article</type><title>Human fetal central nervous system organotypic cultures</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>LYMAN, W. D ; TRICOCHE, M ; HATCH, W. C ; KRESS, Y ; CHIU, F.-C ; RASHBAUM, W. K</creator><creatorcontrib>LYMAN, W. D ; TRICOCHE, M ; HATCH, W. C ; KRESS, Y ; CHIU, F.-C ; RASHBAUM, W. K</creatorcontrib><description>Many aspects of human neurodevelopment remain enigmatic. A main reason for this is, although there have been a significant number of morphologic and biochemical studies of neural tissues derived from human embryos and fetuses, this can only provide a static picture of the events at a given gestational age. Also, in vitro studies that focus on cells derived from these tissues have a limitation in that different cell types in dissociated tissue culture cannot interact in a 'normal' physiologic manner thereby, perhaps, altering their housekeeping and luxury functions. The present study focused on the development of a human explant organotypic culture model that may overcome the static limitation of the first example and permit a dynamic analysis of different cell types as they interact which may satisfy the second restriction. Because there is an array of developmental markers that define different cell phenotypes, this explant model may also provide a means of analyzing, for the first time, processes undefined in the human CNS. Human fetal CNS tissue obtained from second trimester abortuses was established in culture. The tissues were maintained for up to 12 weeks during which time they developed and differentiated. Sample cultures were harvested periodically and analyzed by light- and electron microscopy in combination with immunocytochemistry. Differentiation of neurons, astrocytes, oligodendrocytes and endothelial cells was documented using morphologic and biochemical criteria. As such, this model system may allow for the analysis of processes that occur during normal human fetal neurodevelopment and in pathologic conditions.</description><identifier>ISSN: 0165-3806</identifier><identifier>DOI: 10.1016/0165-3806(91)90044-J</identifier><identifier>PMID: 1893565</identifier><language>eng</language><publisher>Amsterdam: Elsevier</publisher><subject>Biological and medical sciences ; Central Nervous System - cytology ; Central Nervous System - embryology ; Central Nervous System - ultrastructure ; Embryo, Mammalian ; Embryology: invertebrates and vertebrates. Teratology ; Fetus ; Fundamental and applied biological sciences. Psychology ; Gestational Age ; Humans ; Microscopy, Electron ; Nerve Fibers - physiology ; Nerve Fibers - ultrastructure ; Organ Culture Techniques ; Organogenesis. Fetal development ; Organogenesis. Physiological fonctions ; Synapses - physiology ; Synapses - ultrastructure</subject><ispartof>Brain research. Developmental brain research, 1991-06, Vol.60 (2), p.155-160</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-1fa6b8a3c08c907103af94d81b4ae1bad7b1d0b26d89a296f5156041508601953</citedby><cites>FETCH-LOGICAL-c382t-1fa6b8a3c08c907103af94d81b4ae1bad7b1d0b26d89a296f5156041508601953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4959679$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1893565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LYMAN, W. D</creatorcontrib><creatorcontrib>TRICOCHE, M</creatorcontrib><creatorcontrib>HATCH, W. C</creatorcontrib><creatorcontrib>KRESS, Y</creatorcontrib><creatorcontrib>CHIU, F.-C</creatorcontrib><creatorcontrib>RASHBAUM, W. K</creatorcontrib><title>Human fetal central nervous system organotypic cultures</title><title>Brain research. Developmental brain research</title><addtitle>Brain Res Dev Brain Res</addtitle><description>Many aspects of human neurodevelopment remain enigmatic. A main reason for this is, although there have been a significant number of morphologic and biochemical studies of neural tissues derived from human embryos and fetuses, this can only provide a static picture of the events at a given gestational age. Also, in vitro studies that focus on cells derived from these tissues have a limitation in that different cell types in dissociated tissue culture cannot interact in a 'normal' physiologic manner thereby, perhaps, altering their housekeeping and luxury functions. The present study focused on the development of a human explant organotypic culture model that may overcome the static limitation of the first example and permit a dynamic analysis of different cell types as they interact which may satisfy the second restriction. Because there is an array of developmental markers that define different cell phenotypes, this explant model may also provide a means of analyzing, for the first time, processes undefined in the human CNS. Human fetal CNS tissue obtained from second trimester abortuses was established in culture. The tissues were maintained for up to 12 weeks during which time they developed and differentiated. Sample cultures were harvested periodically and analyzed by light- and electron microscopy in combination with immunocytochemistry. Differentiation of neurons, astrocytes, oligodendrocytes and endothelial cells was documented using morphologic and biochemical criteria. As such, this model system may allow for the analysis of processes that occur during normal human fetal neurodevelopment and in pathologic conditions.</description><subject>Biological and medical sciences</subject><subject>Central Nervous System - cytology</subject><subject>Central Nervous System - embryology</subject><subject>Central Nervous System - ultrastructure</subject><subject>Embryo, Mammalian</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Fetus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gestational Age</subject><subject>Humans</subject><subject>Microscopy, Electron</subject><subject>Nerve Fibers - physiology</subject><subject>Nerve Fibers - ultrastructure</subject><subject>Organ Culture Techniques</subject><subject>Organogenesis. Fetal development</subject><subject>Organogenesis. Physiological fonctions</subject><subject>Synapses - physiology</subject><subject>Synapses - ultrastructure</subject><issn>0165-3806</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1Lw0AQxfeg1Fr9DxRyENFDdCb7kd2jFL9KwYuel8lmI5V81N1E6H9vaks9DA_mvTcMP8YuEO4QUN2PI1OuQd0YvDUAQqSLIzY9rE_YaYxfAIBc44RNUBsulZyy_GVoqE0q31OdON_2YdTWh59uiEncxN43SRc-qe36zXrlEjfU_RB8PGPHFdXRn-91xj6eHt_nL-ny7fl1_rBMHddZn2JFqtDEHWhnIEfgVBlRaiwEeSyozAssochUqQ1lRlUSpQKBErQCNJLP2PXu7jp034OPvW1W0fm6ptaPL9o8A2Nyoceg2AVd6GIMvrLrsGoobCyC3TKyWxh2C8MatH-M7GKsXe7vD0Xjy__SDtDoX-19io7qKlDrVvEQE0YalRv-Cy4-cBE</recordid><startdate>19910621</startdate><enddate>19910621</enddate><creator>LYMAN, W. D</creator><creator>TRICOCHE, M</creator><creator>HATCH, W. C</creator><creator>KRESS, Y</creator><creator>CHIU, F.-C</creator><creator>RASHBAUM, W. K</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19910621</creationdate><title>Human fetal central nervous system organotypic cultures</title><author>LYMAN, W. D ; TRICOCHE, M ; HATCH, W. C ; KRESS, Y ; CHIU, F.-C ; RASHBAUM, W. K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-1fa6b8a3c08c907103af94d81b4ae1bad7b1d0b26d89a296f5156041508601953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Biological and medical sciences</topic><topic>Central Nervous System - cytology</topic><topic>Central Nervous System - embryology</topic><topic>Central Nervous System - ultrastructure</topic><topic>Embryo, Mammalian</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Fetus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gestational Age</topic><topic>Humans</topic><topic>Microscopy, Electron</topic><topic>Nerve Fibers - physiology</topic><topic>Nerve Fibers - ultrastructure</topic><topic>Organ Culture Techniques</topic><topic>Organogenesis. Fetal development</topic><topic>Organogenesis. Physiological fonctions</topic><topic>Synapses - physiology</topic><topic>Synapses - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LYMAN, W. D</creatorcontrib><creatorcontrib>TRICOCHE, M</creatorcontrib><creatorcontrib>HATCH, W. C</creatorcontrib><creatorcontrib>KRESS, Y</creatorcontrib><creatorcontrib>CHIU, F.-C</creatorcontrib><creatorcontrib>RASHBAUM, W. K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research. Developmental brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LYMAN, W. D</au><au>TRICOCHE, M</au><au>HATCH, W. C</au><au>KRESS, Y</au><au>CHIU, F.-C</au><au>RASHBAUM, W. K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human fetal central nervous system organotypic cultures</atitle><jtitle>Brain research. Developmental brain research</jtitle><addtitle>Brain Res Dev Brain Res</addtitle><date>1991-06-21</date><risdate>1991</risdate><volume>60</volume><issue>2</issue><spage>155</spage><epage>160</epage><pages>155-160</pages><issn>0165-3806</issn><abstract>Many aspects of human neurodevelopment remain enigmatic. A main reason for this is, although there have been a significant number of morphologic and biochemical studies of neural tissues derived from human embryos and fetuses, this can only provide a static picture of the events at a given gestational age. Also, in vitro studies that focus on cells derived from these tissues have a limitation in that different cell types in dissociated tissue culture cannot interact in a 'normal' physiologic manner thereby, perhaps, altering their housekeeping and luxury functions. The present study focused on the development of a human explant organotypic culture model that may overcome the static limitation of the first example and permit a dynamic analysis of different cell types as they interact which may satisfy the second restriction. Because there is an array of developmental markers that define different cell phenotypes, this explant model may also provide a means of analyzing, for the first time, processes undefined in the human CNS. Human fetal CNS tissue obtained from second trimester abortuses was established in culture. The tissues were maintained for up to 12 weeks during which time they developed and differentiated. Sample cultures were harvested periodically and analyzed by light- and electron microscopy in combination with immunocytochemistry. Differentiation of neurons, astrocytes, oligodendrocytes and endothelial cells was documented using morphologic and biochemical criteria. As such, this model system may allow for the analysis of processes that occur during normal human fetal neurodevelopment and in pathologic conditions.</abstract><cop>Amsterdam</cop><pub>Elsevier</pub><pmid>1893565</pmid><doi>10.1016/0165-3806(91)90044-J</doi><tpages>6</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0165-3806 |
ispartof | Brain research. Developmental brain research, 1991-06, Vol.60 (2), p.155-160 |
issn | 0165-3806 |
language | eng |
recordid | cdi_proquest_miscellaneous_72099748 |
source | MEDLINE; Alma/SFX Local Collection |
subjects | Biological and medical sciences Central Nervous System - cytology Central Nervous System - embryology Central Nervous System - ultrastructure Embryo, Mammalian Embryology: invertebrates and vertebrates. Teratology Fetus Fundamental and applied biological sciences. Psychology Gestational Age Humans Microscopy, Electron Nerve Fibers - physiology Nerve Fibers - ultrastructure Organ Culture Techniques Organogenesis. Fetal development Organogenesis. Physiological fonctions Synapses - physiology Synapses - ultrastructure |
title | Human fetal central nervous system organotypic cultures |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T19%3A51%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Human%20fetal%20central%20nervous%20system%20organotypic%20cultures&rft.jtitle=Brain%20research.%20Developmental%20brain%20research&rft.au=LYMAN,%20W.%20D&rft.date=1991-06-21&rft.volume=60&rft.issue=2&rft.spage=155&rft.epage=160&rft.pages=155-160&rft.issn=0165-3806&rft_id=info:doi/10.1016/0165-3806(91)90044-J&rft_dat=%3Cproquest_cross%3E72099748%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72099748&rft_id=info:pmid/1893565&rfr_iscdi=true |