Phenotypic Diversity of Antigen-Presenting Cells in Ovine-Afferent Intestinal Lymph

The phenotypic and functional repertoire of antigen-presenting cells (APCs) remains incompletely characterized, particularly during the migratory phase of their life history, when these cells leave peripheral tissues and travel via afferent lymphatic vessels to regional lymph nodes. Lymphatic cannul...

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Veröffentlicht in:Archives of medical research 2002-07, Vol.33 (4), p.405-412
Hauptverfasser: Pernthaner, Anton, Cole, Sally-Anne, Gatehouse, Tressa, Hein, Wayne R
Format: Artikel
Sprache:eng
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Zusammenfassung:The phenotypic and functional repertoire of antigen-presenting cells (APCs) remains incompletely characterized, particularly during the migratory phase of their life history, when these cells leave peripheral tissues and travel via afferent lymphatic vessels to regional lymph nodes. Lymphatic cannulation procedures were used to collect ovine APCs as they migrated from the mucosa of the small intestine to regional lymph nodes. A panel of 19 new monoclonal antibodies (mAbs) was produced to characterize surface molecules expressed on APCs by means of two-color flow cytometry and microscopy. Two broad patterns of mAb reactivity were evident. Twelve mAbs reacted almost exclusively with cells in the APC-gated region, because all these mAbs stained
ISSN:0188-4409
1873-5487
DOI:10.1016/S0188-4409(02)00375-2