Rapid Enzymatic Method for the Measurement of Mannitol in Urine

A previously described method for mannitol in urine has been modified and improved. End product inhibition by fructose in the mannitol dehydrogenase method for mannitol has been minimized; the assay is linear over a sample mannitol concentration range of 0–12 mmol/L; no significant interference from...

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Veröffentlicht in:	Annals of clinical biochemistry 1991-07, Vol.28 (4), p.401-406
Hauptverfasser:	Blood, Jane, Ingle, A R, Allison, N, Davies, G R, Hill, P G
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Carbohydrates Chromatography, Gas Clinical Medicine - methods Fundamental and applied biological sciences. Psychology Hexokinase - pharmacology Holosides Hydrogen-Ion Concentration Mannitol - urine Mannitol Dehydrogenases Other biological molecules
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Beschreibung
Zusammenfassung:	A previously described method for mannitol in urine has been modified and improved. End product inhibition by fructose in the mannitol dehydrogenase method for mannitol has been minimized; the assay is linear over a sample mannitol concentration range of 0–12 mmol/L; no significant interference from other sugars or sugar alcohols could be demonstrated. The method is precise (within-batch CV < 1%), rapid and shows excellent recovery of mannitol in spiked samples. Comparison with gas liquid chromatography shows excellent correlation (r = 0 · 994) between the two methods.
ISSN:	0004-5632 1758-1001
DOI:	10.1177/000456329102800416