Localization of a VP3 epitope of infectious bursal disease virus

An immunodominant region of VP3, one of the two structural proteins of infectious bursal disease virus (IBDV strain 002-73), has been mapped by restriction site-specific deletion analysis and subcloning in Escherichia coli, followed by immunoblot analysis of the synthesized products. The epitope loc...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Virology (New York, N.Y.) N.Y.), 1991-10, Vol.184 (2), p.805-807
Hauptverfasser:	Jagadish, Mittur N., Azad, Ahmed A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES ANTIGENE ANTIGENOS Antigens, Viral - genetics Antigens, Viral - immunology AVES DE CORRAL Biological and medical sciences Capsid - genetics Capsid - immunology Capsid Proteins Cloning, Molecular DNA Mutational Analysis Epitopes Fundamental and applied biological sciences. Psychology IMMUNODIAGNOSTIC INFECCION INFECTION Infectious bursal disease virus - genetics Infectious bursal disease virus - immunology INMUNODIAGNOSTICO Microbiology Molecular Sequence Data Morphology, structure, chemical composition, physicochemical properties PROTEINAS PROTEINE Proteins - genetics Proteins - immunology Restriction Mapping Virology VIRUS VIRUS BRONCHITE INFECTIEUSE AVIAIRE VIRUS BRONQUITIS INFECCIOSA AVIAR VOLAILLE
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	An immunodominant region of VP3, one of the two structural proteins of infectious bursal disease virus (IBDV strain 002-73), has been mapped by restriction site-specific deletion analysis and subcloning in Escherichia coli, followed by immunoblot analysis of the synthesized products. The epitope located within 58 amino acids reacted very strongly with a mouse monoclonal antibody (MAb 17 80 ) raised against lBDV 002-73. This immunodominant region may be useful in serodiagnosis of IBDV infection in poultry.
ISSN:	0042-6822 1096-0341
DOI:	10.1016/0042-6822(91)90457-M