Peanut agglutinin as a marker of maturation and activation of chicken thymic-derived lymphocytes

In mammals peanut agglutinin (PNA) is used for separation of lymphocytes into immature and mature T-cells. Lymphocytes that bind PNA are young and functionally immature, but the cells that do not bind PNA are considered mature. No conclusive information was published on PNA as a marker of lymphocyte...

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Veröffentlicht in:Poultry science 1991-07, Vol.70 (7), p.1516-1520
Hauptverfasser: Uni, Z. (The Hebrew University of Jerusalem, Rehovot, Israel), Heller, E.D
Format: Artikel
Sprache:eng
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Zusammenfassung:In mammals peanut agglutinin (PNA) is used for separation of lymphocytes into immature and mature T-cells. Lymphocytes that bind PNA are young and functionally immature, but the cells that do not bind PNA are considered mature. No conclusive information was published on PNA as a marker of lymphocytes maturation in chickens. The present results showed that T-lymphocytes that do not bind PNA (PNA-) responded significantly higher to Concanavalin A (Con-A) stimulation than PNA+ ones, During Con-A activation, a PNA receptor was revealed. Double staining of Con-A cultured lymphocytes revealed the presence of both I-associated antigen and PNA. Therefore, the PNA receptor, on chicken T-lymphocyte, was not indicative always of immaturity. During maturation, the receptor was covered and the cells were PNA-, but subsequent proliferation reverted PNA- cells to PNA+ cells
ISSN:0032-5791
1525-3171
DOI:10.3382/ps.0701516