Functional cloning of a Dictyostelium discoideum cDNA encoding GMP synthetase

A functional cloning procedure has been used to recover a cDNA coding for the GMP synthetase of Dictyostelium discoideum. The enzyme is encoded by a single gene, which is actively transcribed during growth, but not during development. The open reading frame encodes a protein of 718 amino acids with...

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Veröffentlicht in:	The Journal of biological chemistry 1991-09, Vol.266 (25), p.16448-16452
Hauptverfasser:	Van Lookeren Campagne, M.M. (Columbia University, New York, NY), Franke, J, Kessin, R.H
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN Amino Acid Sequence Base Sequence Biological and medical sciences Blotting, Northern Blotting, Southern Carbon-Nitrogen Ligases CLONACION CLONAGE Cloning, Molecular Dictyostelium - enzymology Dictyostelium - genetics Dictyostelium discoideum DNA, Fungal Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology genes Genes. Genome LIGASAS LIGASE Ligases - genetics Ligases - metabolism MIXOMICETES Molecular and cellular biology Molecular genetics Molecular Sequence Data MYXOMYCETES NUCLEOTIDE NUCLEOTIDOS Sequence Alignment
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Zusammenfassung:	A functional cloning procedure has been used to recover a cDNA coding for the GMP synthetase of Dictyostelium discoideum. The enzyme is encoded by a single gene, which is actively transcribed during growth, but not during development. The open reading frame encodes a protein of 718 amino acids with a predicted molecular mass of 79.6 kDa. The Dictyotelium enzyme has extensive homology with the GMP synthetase of Escherichia coli and regional homology to other glutamine amidotransferases
ISSN:	0021-9258 1083-351X
DOI:	10.1016/S0021-9258(18)55320-5