Zymographic detection of cinnamic acid decarboxylase activity
The manuscript includes a concise description of a new, fast and simple method for detection of cinnamic acid decarboxylase activity. The method is based on a color shift caused a by pH change and may be an excellent procedure for large screenings of samples from natural sources, as it involves no c...
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| Veröffentlicht in: | Journal of microbiological methods 2002-11, Vol.51 (3), p.417-420 |
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| container_end_page | 420 |
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| container_issue | 3 |
| container_start_page | 417 |
| container_title | Journal of microbiological methods |
| container_volume | 51 |
| creator | Prim, Núria Pastor, F.I.Javier Diaz, Pilar |
| description | The manuscript includes a concise description of a new, fast and simple method for detection of cinnamic acid decarboxylase activity. The method is based on a color shift caused a by pH change and may be an excellent procedure for large screenings of samples from natural sources, as it involves no complex sample processing or purification. The method developed can be used in preliminary approaches to biotransformation processes involving detection of hydroxycinnamic acid decarboxylase activity. |
| doi_str_mv | 10.1016/S0167-7012(02)00109-4 |
| format | Article |
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The method is based on a color shift caused a by pH change and may be an excellent procedure for large screenings of samples from natural sources, as it involves no complex sample processing or purification. The method developed can be used in preliminary approaches to biotransformation processes involving detection of hydroxycinnamic acid decarboxylase activity.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/S0167-7012(02)00109-4</identifier><identifier>PMID: 12223304</identifier><identifier>CODEN: JMIMDQ</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Bacillus - enzymology ; Bacteriological methods and techniques used in bacteriology ; Bacteriological Techniques ; Bacteriology ; Biological and medical sciences ; Bromcresol Purple - metabolism ; Carboxy-Lyases - chemistry ; Carboxy-Lyases - metabolism ; Cinnamates - metabolism ; Culture Media ; Decarboxylase activity ; Electrophoresis, Polyacrylamide Gel - methods ; Escherichia coli - enzymology ; Fundamental and applied biological sciences. 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The method developed can be used in preliminary approaches to biotransformation processes involving detection of hydroxycinnamic acid decarboxylase activity.</description><subject>Bacillus - enzymology</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriological Techniques</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Bromcresol Purple - metabolism</subject><subject>Carboxy-Lyases - chemistry</subject><subject>Carboxy-Lyases - metabolism</subject><subject>Cinnamates - metabolism</subject><subject>Culture Media</subject><subject>Decarboxylase activity</subject><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>Escherichia coli - enzymology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydroxycinnamic acids</subject><subject>Microbiology</subject><subject>Vanillin</subject><subject>Zymogram</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAQgIMo7rr6E5S9KHqo5tUmOYjI4gsWPKgXLyFNE420zZp0F_vvTd1Fj8IwA5NvkskHwCGC5wii4uIpJZYxiPApxGcQIigyugXGiDOccZKLbTD-RUZgL8aPBOWE8l0wQhhjQiAdg8vXvvFvQS3enZ5WpjO6c76dejvVrm1Vk7pKuyodaRVK_9XXKprU6tzKdf0-2LGqjuZgUyfg5fbmeXafzR_vHmbX80zTnHWZRgVktBQCVby0JSKpGlpaQW3OlLZpE6g4tCRnPDe4EqrQ2lSVgFRhUXIyASfrexfBfy5N7GTjojZ1rVrjl1EyDBnhRZHAfA3q4GMMxspFcI0KvURQDt7kjzc5SJEwxeBN0jR3tHlgWTam-pvaiErA8QZQUavaBtVqF_84IgqB8MBdrTmTdKycCTJqZ9r0GxeSW1l5988q38WTibM</recordid><startdate>20021101</startdate><enddate>20021101</enddate><creator>Prim, Núria</creator><creator>Pastor, F.I.Javier</creator><creator>Diaz, Pilar</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20021101</creationdate><title>Zymographic detection of cinnamic acid decarboxylase activity</title><author>Prim, Núria ; Pastor, F.I.Javier ; Diaz, Pilar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-c16074b991d8bfb131d8e4bf94f57acf3040a80f35785e2d9a6ccedd904a29b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Bacillus - enzymology</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriological Techniques</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Bromcresol Purple - metabolism</topic><topic>Carboxy-Lyases - chemistry</topic><topic>Carboxy-Lyases - metabolism</topic><topic>Cinnamates - metabolism</topic><topic>Culture Media</topic><topic>Decarboxylase activity</topic><topic>Electrophoresis, Polyacrylamide Gel - methods</topic><topic>Escherichia coli - enzymology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydroxycinnamic acids</topic><topic>Microbiology</topic><topic>Vanillin</topic><topic>Zymogram</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prim, Núria</creatorcontrib><creatorcontrib>Pastor, F.I.Javier</creatorcontrib><creatorcontrib>Diaz, Pilar</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prim, Núria</au><au>Pastor, F.I.Javier</au><au>Diaz, Pilar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Zymographic detection of cinnamic acid decarboxylase activity</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2002-11-01</date><risdate>2002</risdate><volume>51</volume><issue>3</issue><spage>417</spage><epage>420</epage><pages>417-420</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><coden>JMIMDQ</coden><abstract>The manuscript includes a concise description of a new, fast and simple method for detection of cinnamic acid decarboxylase activity. 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| fulltext | fulltext |
| identifier | ISSN: 0167-7012 |
| ispartof | Journal of microbiological methods, 2002-11, Vol.51 (3), p.417-420 |
| issn | 0167-7012 1872-8359 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Bacillus - enzymology Bacteriological methods and techniques used in bacteriology Bacteriological Techniques Bacteriology Biological and medical sciences Bromcresol Purple - metabolism Carboxy-Lyases - chemistry Carboxy-Lyases - metabolism Cinnamates - metabolism Culture Media Decarboxylase activity Electrophoresis, Polyacrylamide Gel - methods Escherichia coli - enzymology Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Hydroxycinnamic acids Microbiology Vanillin Zymogram |
| title | Zymographic detection of cinnamic acid decarboxylase activity |
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