Zymographic detection of cinnamic acid decarboxylase activity

The manuscript includes a concise description of a new, fast and simple method for detection of cinnamic acid decarboxylase activity. The method is based on a color shift caused a by pH change and may be an excellent procedure for large screenings of samples from natural sources, as it involves no c...

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Veröffentlicht in:	Journal of microbiological methods 2002-11, Vol.51 (3), p.417-420
Hauptverfasser:	Prim, Núria, Pastor, F.I.Javier, Diaz, Pilar
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus - enzymology Bacteriological methods and techniques used in bacteriology Bacteriological Techniques Bacteriology Biological and medical sciences Bromcresol Purple - metabolism Carboxy-Lyases - chemistry Carboxy-Lyases - metabolism Cinnamates - metabolism Culture Media Decarboxylase activity Electrophoresis, Polyacrylamide Gel - methods Escherichia coli - enzymology Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Hydroxycinnamic acids Microbiology Vanillin Zymogram
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Beschreibung
Zusammenfassung:	The manuscript includes a concise description of a new, fast and simple method for detection of cinnamic acid decarboxylase activity. The method is based on a color shift caused a by pH change and may be an excellent procedure for large screenings of samples from natural sources, as it involves no complex sample processing or purification. The method developed can be used in preliminary approaches to biotransformation processes involving detection of hydroxycinnamic acid decarboxylase activity.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/S0167-7012(02)00109-4