Generation of transgenic dairy cattle using 'in vitro' embryo production

We have combined gene transfer, by microinjection, with ‘ in vitro ’ embryo production technology, enabling us to carry out non-surgical transfer, to recipient cows, of microinjected embryos that have been cultured from immature oocytes. Using this approach, we have established 21 pregnancies from w...

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Veröffentlicht in:	Bio/Technology 1991-09, Vol.9 (9), p.844-847
Hauptverfasser:	Krimpenfort, P. (Gene Pharming Europe B.V., Leiden, The Netherlands), Rademakers, A, Eyestone, W, Schans, A. van der, Broek, S. van den, Kooiman, P, Kootwijk, E, Platenburg, G, Pieper, F, Stijker, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture ANIMAL TRANSGENIQUE ANIMALES TRANSGENICOS Animals Animals, Genetically Modified BINDING PROTEINS Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Blotting, Southern BOVIN LAITIER Caseins - genetics Cattle - genetics Cell fusion (prokaryotes) Cells, Cultured DAIRY CATTLE DNA - genetics DNA - isolation & purification EMBRYO TRANSFER Embryonic and Fetal Development EXPERIMENTACION IN VITRO EXPERIMENTATION IN VITRO Female Fundamental and applied biological sciences. Psychology GANADO DE LECHE GENE TRANSFER Genetic engineering Genetic technics Humans IN VITRO EXPERIMENTATION LACTOFERRIN Lactoferrin - genetics LACTOFERRINAS LACTOFERRINE Life Sciences Methods. Procedures. Technologies microinjection Oocytes - physiology Pregnancy protein expression PROTEINAS PROTEINE PROTEINS research-paper Restriction Mapping Transfection TRANSFERENCIA DE EMBRIONES TRANSFERENCIA DE GENES TRANSFERT DE GENE TRANSFERT EMBRYONNAIRE TRANSGENIC ANIMALS
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Beschreibung
Zusammenfassung:	We have combined gene transfer, by microinjection, with ‘ in vitro ’ embryo production technology, enabling us to carry out non-surgical transfer, to recipient cows, of microinjected embryos that have been cultured from immature oocytes. Using this approach, we have established 21 pregnancies from which 19 calves were born. Southern blot analysis proved that in two cases the microinjected DNA had been integrated in the host genome.
ISSN:	0733-222X 1087-0156 2331-3684 1546-1696
DOI:	10.1038/nbt0991-844