Preparation and analysis of small unilamellar phospholipid vesicles of a uniform size

Preparation and analysis of small unilamellar phospholipid vesicles of a uniform size

The interaction of carbonmonoxyhemoglobin and heme with small unilamellar phospholipid vesicles was studied using dynamic light scattering. Addition of carbonmonoxyhemoglobin to dimyristoylphosphatidylcholine:dimyristoylphosphatidylserine small unilamellar vesicles resulted in an increase of average...

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Veröffentlicht in:Biochemical and biophysical research communications 2002-09, Vol.296 (5), p.1352-1355
Hauptverfasser: Pitcher III, Wayne H, Huestis, Wray H
Format: Artikel
Sprache:eng
Schlagworte:
Carboxyhemoglobin - chemistry
Dimyristoylphosphatidylcholine - analysis
Dynamic light scattering
Heme - chemistry
Hemoglobin
Light
Lipid vesicles
Liposomes - chemistry
Phosphatidylserines - analysis
Phospholipids - analysis
Scattering, Radiation
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Zusammenfassung:The interaction of carbonmonoxyhemoglobin and heme with small unilamellar phospholipid vesicles was studied using dynamic light scattering. Addition of carbonmonoxyhemoglobin to dimyristoylphosphatidylcholine:dimyristoylphosphatidylserine small unilamellar vesicles resulted in an increase of average vesicle size from 17.4 to 32.0 nm. Addition of heme to vesicles produced a smaller size increase, from 17.4 to 21.0 nm. Also reported is a method for preparing small unilamellar lipid vesicles of a uniform size, suitable for use in NMR spectroscopy.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(02)02092-2