Development and testing of a packaging cell line for avian retroviral vectors

A new helper cell line designated L3.07, has been used to package spleen necrosis virus (SNV)-based vectors, resulting in the production of high titres of replication defective retroviruses. One of these vectors, vSNO21 has been shown to infect avian primordial germ cells (PGCs).

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Bibliographische Detailangaben
Veröffentlicht in:	Archives of virology 1991-01, Vol.119 (3/4), p.257-264
Hauptverfasser:	Meyers, N.L, Booth, S.C, Bumstead, N, Vick, L, Simkiss, K
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Biotechnology Cell Line cell lines chickens Coturnix Fibroblasts Fundamental and applied biological sciences. Psychology gene transfer Genetic engineering Genetic technics Genetic Vectors genetically modified organisms germ cells helper cell lines Methods. Procedures. Technologies Mice Plasmids replication Restriction Mapping retroviral vectors Retroviridae Simian virus 40 - genetics Simian virus 40 - ultrastructure spleen necrosis virus Vectors (cloning, transfer, expression). Insertion sequences and transposons Viral Structural Proteins - genetics Virus Replication
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Beschreibung
Zusammenfassung:	A new helper cell line designated L3.07, has been used to package spleen necrosis virus (SNV)-based vectors, resulting in the production of high titres of replication defective retroviruses. One of these vectors, vSNO21 has been shown to infect avian primordial germ cells (PGCs).
ISSN:	0304-8608 1432-8798
DOI:	10.1007/BF01310674