Gallid herpesvirus 1 (infectious laryngotracheitis virus): cloning and physical maps of the SA-2 strain

Clones representing 90% of the genome of Gallid herpesvirus 1 (infectious laryngotracheitis virus; ILTV) were obtained and used in hybridization experiments to construct EcoRI, KpnI and SmaI physical maps. The genome was 155 kilobase pairs (kbp) and comprised of a long unique sequence (120 kbp) and...

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Veröffentlicht in:	Archives of virology 1991-01, Vol.119 (3/4), p.181-198
Hauptverfasser:	Johnson, M.A, Prideaux, C.T, Kongsuwan, K, Sheppard, M, Fahey, K.J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cells, Cultured Chickens clones cloning Cloning, Molecular Deoxyribonuclease EcoRI Deoxyribonucleases, Type II Site-Specific DNA DNA, Viral Fundamental and applied biological sciences. Psychology gallid herpesvirus 1 Genes, Viral Genes. Genome genome Herpesvirus 1, Gallid - genetics Iltovirus Molecular and cellular biology Molecular genetics nucleotide sequences Repetitive Sequences, Nucleic Acid Restriction Mapping Viral Vaccines - genetics
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Beschreibung
Zusammenfassung:	Clones representing 90% of the genome of Gallid herpesvirus 1 (infectious laryngotracheitis virus; ILTV) were obtained and used in hybridization experiments to construct EcoRI, KpnI and SmaI physical maps. The genome was 155 kilobase pairs (kbp) and comprised of a long unique sequence (120 kbp) and a short unique sequence (17 kbp) bounded by repeat sequences each of 9 kbp. An unrelated second pair of repeat sequences was located at 0.67 and 0.88 map untis. A terminal repeat of the unique long region (UL) was also detected, but no isomerization of UL was detected.
ISSN:	0304-8608 1432-8798
DOI:	10.1007/BF01310669