Defense chemistry of cyanogenic Eucalyptus cladocalyx seedlings is affected by water supply

Cyanogenesis is a widespread and effective defense mechanism in plants. Published evidence suggests that cyanogenic capacity (i.e., cyanogenic glycoside concentration) is enhanced in response to water stress, although potentially confounding variables preclude a definite conclusion. We used highly cyanogenic Eucalyptus cladocalyx var. nana F. Muell. seedlings grown with varying amounts of water and nitrogen (N) to determine the relationship between cyanogenic capacity and water stress. We also examined whether variation in cyanogenic capacity affects phenolic biosynthesis because both pathways use phenylalanine as a substrate. Cyanogenic capacity in fully expanded leaves increased 70% in response to moderate water stress when N availability was high but only 30% when growth was N-limited. Absolute cyanogenic capacity also increased with increasing N supply. Total phenolics and condensed tannins decreased with increasing N supply, but these compounds were unaffected by water stress. We conclude that, under the influence of water stress, the enhanced demand for phenylalanine for cyanogenic glycoside biosynthesis can be sustained by enhanced shikimate pathway flux without affecting phenolic metabolism.