Atmospheric pressure laser desorption/ionization on porous silicon
A recently developed commercial atmospheric pressure matrix‐assisted laser desorption/ionization (AP‐MALDI) source (MassTech, Inc.) was modified to adopt commercially available DIOS plates (Mass Consortium Corp.) for the studies of laser desorption from the surface of porous silicon under atmospheri...
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Veröffentlicht in: | Rapid communications in mass spectrometry 2002-01, Vol.16 (18), p.1737-1742 |
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Sprache: | eng |
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Zusammenfassung: | A recently developed commercial atmospheric pressure matrix‐assisted laser desorption/ionization (AP‐MALDI) source (MassTech, Inc.) was modified to adopt commercially available DIOS plates (Mass Consortium Corp.) for the studies of laser desorption from the surface of porous silicon under atmospheric pressure conditions. The feasibility of atmospheric pressure laser desorption/ionization from the surface of porous silicon (AP‐DIOS) was demonstrated. The advantages of this new AP‐DIOS technique include reasonably good sensitivity (subpicomole range for standard peptide mixtures), simplicity of sample preparation, uniformity of target spots and the absence of matrix peaks in the spectra. The AP‐DIOS source was interfaced with a commercial ion trap (LCQ Classic, Thermo Finnigan) which additionally provides a unique MSn capability. The AP‐DIOS spectrum of 250 fmol of unseparated tryptic digest of bovine serum albumin (BSA) was compared with that of AP‐MALDI for the same compound. AP‐DIOS offers significantly better coverage for the digest components in the mass range 200–1000 Da. The combined data of both techniques enabled us to nearly double the number of matched peaks in BSA digest analysis compared with AP‐DIOS or AP‐MALDI analysis separately. Copyright © 2002 John Wiley & Sons, Ltd. |
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ISSN: | 0951-4198 1097-0231 |
DOI: | 10.1002/rcm.781 |