Determination of xylanase, beta-glucanase, and cellulase activity

A simple, robust and highly reproducible method for the determination of xylanase, beta-glucanase, and cellulase in commercial feed enzyme preparations is described. The method is based on measurement of reducing moieties released by the enzymes from arabinoxylan, beta-glucan, or carboxymethylcellul...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2002-09, Vol.374 (1), p.80-87
Hauptverfasser:	König, Joachim, Grasser, Roland, Pikor, Heather, Vogel, Kurt
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Feed Cellulase - analysis Cellulase - metabolism Glycoside Hydrolases - analysis Glycoside Hydrolases - metabolism Kinetics Reproducibility of Results Sensitivity and Specificity Substrate Specificity Xylan Endo-1,3-beta-Xylosidase Xylosidases - analysis Xylosidases - metabolism
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Beschreibung
Zusammenfassung:	A simple, robust and highly reproducible method for the determination of xylanase, beta-glucanase, and cellulase in commercial feed enzyme preparations is described. The method is based on measurement of reducing moieties released by the enzymes from arabinoxylan, beta-glucan, or carboxymethylcellulose (CMC) and is independent of enzyme standards.
ISSN:	1618-2642 1618-2650
DOI:	10.1007/s00216-002-1379-7