Fluorescence Enhancement through Enzymatic Cleavage of Internally Quenched Dendritic Peptides: A Sensitive Assay for the AspN Endoproteinase

Fluorescence Enhancement through Enzymatic Cleavage of Internally Quenched Dendritic Peptides: A Sensitive Assay for the AspN Endoproteinase

A way to reduce the synthetic work associated with FRET‐based techniques for protease analysis and characterization is shown here. Protease‐mediated cleavage of fluorescent peptides attached to dendrimers results in large increases in fluorescence (see scheme).

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Bibliographische Detailangaben
Veröffentlicht in:Angewandte Chemie (International ed.) 2002-09, Vol.41 (17), p.3233-3236
Hauptverfasser: Ellard, John M, Zollitsch, Thomas, Cummins, W. Jon, Hamilton, Alan L, Bradley, Mark
Format: Artikel
Sprache:eng
Schlagworte:
Chromatography, High Pressure Liquid
Chymotrypsin - chemistry
Dendrimers
Endopeptidases - chemistry
fluorescence
Fluorescent Dyes
Kinetics
lyases
Metalloendopeptidases
peptides
Peptides - chemical synthesis
Peptides - chemistry
Polyamines
solid-phase synthesis
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Beschreibung
Zusammenfassung:A way to reduce the synthetic work associated with FRET‐based techniques for protease analysis and characterization is shown here. Protease‐mediated cleavage of fluorescent peptides attached to dendrimers results in large increases in fluorescence (see scheme).
ISSN:1433-7851
1521-3773
DOI:10.1002/1521-3773(20020902)41:17<3233::AID-ANIE3233>3.0.CO;2-E