Substrate Arrays as Enzyme Fingerprinting Tools

Portrait of an enzyme: Enzyme assays are essential tools of enzymology. Profiling enzyme activities over parameter ranges such as different pH and temperature values forms the basis of mechanistic studies. The comparison of enzyme activities for different substrates delivers activity profiles that a...

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Veröffentlicht in:Chembiochem : a European journal of chemical biology 2002-08, Vol.3 (8), p.701-708
Hauptverfasser: Reymond, Jean-Louis, Wahler, Denis
Format: Artikel
Sprache:eng
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Zusammenfassung:Portrait of an enzyme: Enzyme assays are essential tools of enzymology. Profiling enzyme activities over parameter ranges such as different pH and temperature values forms the basis of mechanistic studies. The comparison of enzyme activities for different substrates delivers activity profiles that are essential for the practical use of enzymes. Fluorogenic or chromogenic substrates can be used to record enzyme activity for several substrates in parallel and the results can be displayed as 2‐dimensional arrays of gray‐scale squares that form an enzyme fingerprint. Enzymes can also be profiled by active‐site‐directed enzyme probes such as alkylating agents and the labeled enzymes can be visualized on electrophoretic gels. Enzyme profiling and fingerprinting techniques are likely to become useful in enzyme discovery and quality control applications.
ISSN:1439-4227
1439-7633
DOI:10.1002/1439-7633(20020802)3:8<701::AID-CBIC701>3.0.CO;2-3