Direct detection of Pneumocystis carinii in fresh bronchoalveolar lavage specimens

Rapid, sensitive and specific methods for the diagnosis of Pneumocystis carinii pneumonia and early institution of therapy are necessary to improve the prognosis of infected patients. Bronchoalveolar lavage (BAL) or sputum induction should be used to obtain adequate material for the identification o...

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Veröffentlicht in:European journal of clinical microbiology & infectious diseases 1991-05, Vol.10 (5), p.460-462
Hauptverfasser: Carmello, P, Giacobbi, D, Savoia, D, Sinicco, A, Gioannini, P
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Sprache:eng
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Zusammenfassung:Rapid, sensitive and specific methods for the diagnosis of Pneumocystis carinii pneumonia and early institution of therapy are necessary to improve the prognosis of infected patients. Bronchoalveolar lavage (BAL) or sputum induction should be used to obtain adequate material for the identification of Pneumocystis carinii . At least 20-30 ml of BAL were centrifuged at 200 x g for 15 min. Digestion of the specimen was performed only in the case of mucinous material (Sputasol, Oxoid, UK). The pellet was used to prepare standard glass slides (4-5 ml of BAL/slide on average) for wet mount preparation and standard stains (Giemsa, Gomori methenamine silver, toluidine blue O) and indirect immunofluorescence.
ISSN:0934-9723
DOI:10.1007/BF01968032