A Bacterial Small-Molecule Three-Hybrid System

A Bacterial Small-Molecule Three-Hybrid System

A method for in vivo affinity chromatography, the yeast three‐hybrid assay simplifies protein identification and amplification at the end of affinity panning. The bacterial system described should increase, by several orders of magnitude, the number of protein variants that can be assayed (see pictu...

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Veröffentlicht in:Angewandte Chemie International Edition 2002-07, Vol.41 (13), p.2327-2330
Hauptverfasser: Althoff, Eric A., Cornish, Virginia W.
Format: Artikel
Sprache:eng
Schlagworte:
bioorganic chemistry
DNA-Directed RNA Polymerases - metabolism
Escherichia coli - genetics
Escherichia coli - metabolism
gene expression
Methotrexate - metabolism
Molecular Structure
Protein Binding
protein engineering
proteomics
signal transduction
Tacrolimus - analogs & derivatives
Tacrolimus - metabolism
Tacrolimus Binding Protein 1A - metabolism
Tetrahydrofolate Dehydrogenase - metabolism
Transcription, Genetic
Two-Hybrid System Techniques
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Beschreibung
Zusammenfassung:A method for in vivo affinity chromatography, the yeast three‐hybrid assay simplifies protein identification and amplification at the end of affinity panning. The bacterial system described should increase, by several orders of magnitude, the number of protein variants that can be assayed (see picture; SLF = synthetic analogue of FK506; Mtx = methotrexate; FKBP12 = FK506‐binding protein 12; DHFR = dihydrofolate reductase; λcI = λ‐repressor; αNTD = N‐terminal domain of the α‐subunit of RNA Pol; RNA Pol = RNA polymerase).
ISSN:1433-7851
1521-3773
DOI:10.1002/1521-3773(20020703)41:13<2327::AID-ANIE2327>3.0.CO;2-U