Monitoring Molecular Recognition of the Ribosomal Decoding Site

Adenine flips out: A combination of X‐ray crystallography, 2‐aminopurine fluorescence labeling, and the use of aminoglycosides as ligands is exploited to demonstrate conformational transitions in the RNA domain that ensures accurate protein synthesis (see picture). The triggering of a conformational...

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Veröffentlicht in:	Angewandte Chemie International Edition 2004-06, Vol.43 (24), p.3177-3182
Hauptverfasser:	Shandrick, Sarah, Zhao, Qiang, Han, Qing, Ayida, Benjamin K., Takahashi, Masayuki, Winters, Geoffrey C., Simonsen, Klaus B., Vourloumis, Dionisios, Hermann, Thomas
Format:	Artikel
Sprache:	eng
Schlagworte:	2-Aminopurine - chemistry Aminoglycosides - chemical synthesis Anti-Bacterial Agents - chemical synthesis antibiotics Crystallization Crystallography, X-Ray fluorescent probes Gene Targeting Models, Molecular molecular recognition Nucleic Acid Conformation Oligonucleotides - chemistry Ribosomes - chemistry Ribosomes - drug effects RNA structures RNA, Bacterial - biosynthesis RNA, Bacterial - chemistry RNA, Bacterial - drug effects Spectrometry, Fluorescence Spectrophotometry, Ultraviolet structure elucidation
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Zusammenfassung:	Adenine flips out: A combination of X‐ray crystallography, 2‐aminopurine fluorescence labeling, and the use of aminoglycosides as ligands is exploited to demonstrate conformational transitions in the RNA domain that ensures accurate protein synthesis (see picture). The triggering of a conformational change of an adenine unit in the RNA by ligand binding can be used as the basis of a screening method to discover antibiotics.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.200454217