Development of PCR markers for the Pl5/Pl8 locus for resistance to Plasmopara halstedii in sunflower, Helianthus annuus L. from complete CC-NBS-LRR sequences
Sunflower downy mildew, caused by Plasmopara halstedii, is one of the major diseases of this crop. Development of elite sunflower lines resistant to different races of this oomycete seems to be the most efficient method to limit downy mildew damage. At least two different gene clusters conferring re...
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| Veröffentlicht in: | Theoretical and applied genetics 2004-06, Vol.109 (1), p.176-185 |
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| creator | Radwan, O Bouzidi, M.F Nicolas, P Mouzeyar, S |
| description | Sunflower downy mildew, caused by Plasmopara halstedii, is one of the major diseases of this crop. Development of elite sunflower lines resistant to different races of this oomycete seems to be the most efficient method to limit downy mildew damage. At least two different gene clusters conferring resistance to different races of P. halstedii have been described. In this work we report the cloning and mapping of two full-length resistance gene analogs (RGA) belonging to the CC-NBC-LRR class of plant resistance genes. The two sequences were then used to develop 14 sequence tagged sites (STS) within the Pl5/Pl8 locus conferring resistance to a wide range of P. halstedii races. These STSs will be useful in marker-assisted selection programs. |
| doi_str_mv | 10.1007/s00122-004-1613-0 |
| format | Article |
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Development of elite sunflower lines resistant to different races of this oomycete seems to be the most efficient method to limit downy mildew damage. At least two different gene clusters conferring resistance to different races of P. halstedii have been described. In this work we report the cloning and mapping of two full-length resistance gene analogs (RGA) belonging to the CC-NBC-LRR class of plant resistance genes. The two sequences were then used to develop 14 sequence tagged sites (STS) within the Pl5/Pl8 locus conferring resistance to a wide range of P. halstedii races. These STSs will be useful in marker-assisted selection programs.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s00122-004-1613-0</identifier><identifier>PMID: 15007505</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Amino Acid Sequence ; amino acid sequences ; artificial selection ; Base Sequence ; Blotting, Southern ; Chromosome Mapping ; complementary DNA ; Crop diseases ; disease resistance ; DNA Primers ; DNA, Complementary - genetics ; downy mildew ; genes ; genetic markers ; Genetic Markers - genetics ; genetic resistance ; Genetics ; Helianthus - genetics ; Helianthus annuus ; Immunity, Innate - genetics ; loci ; marker-assisted selection ; Molecular Sequence Data ; Nucleic Acid Amplification Techniques ; nucleotide sequences ; Oomycetes ; plant breeding ; Plant Diseases - genetics ; Plant Diseases - microbiology ; plant pathogenic fungi ; Plasmopara halstedii ; Polymerase Chain Reaction ; resistance gene analogs ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Tagged Sites</subject><ispartof>Theoretical and applied genetics, 2004-06, Vol.109 (1), p.176-185</ispartof><rights>Springer-Verlag 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c280t-fa03138524ae89512a56352e7f84c777fd4e0df8f771ff1059506cae549062fc3</citedby><cites>FETCH-LOGICAL-c280t-fa03138524ae89512a56352e7f84c777fd4e0df8f771ff1059506cae549062fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15007505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Radwan, O</creatorcontrib><creatorcontrib>Bouzidi, M.F</creatorcontrib><creatorcontrib>Nicolas, P</creatorcontrib><creatorcontrib>Mouzeyar, S</creatorcontrib><title>Development of PCR markers for the Pl5/Pl8 locus for resistance to Plasmopara halstedii in sunflower, Helianthus annuus L. from complete CC-NBS-LRR sequences</title><title>Theoretical and applied genetics</title><addtitle>Theor Appl Genet</addtitle><description>Sunflower downy mildew, caused by Plasmopara halstedii, is one of the major diseases of this crop. Development of elite sunflower lines resistant to different races of this oomycete seems to be the most efficient method to limit downy mildew damage. At least two different gene clusters conferring resistance to different races of P. halstedii have been described. In this work we report the cloning and mapping of two full-length resistance gene analogs (RGA) belonging to the CC-NBC-LRR class of plant resistance genes. The two sequences were then used to develop 14 sequence tagged sites (STS) within the Pl5/Pl8 locus conferring resistance to a wide range of P. halstedii races. These STSs will be useful in marker-assisted selection programs.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>artificial selection</subject><subject>Base Sequence</subject><subject>Blotting, Southern</subject><subject>Chromosome Mapping</subject><subject>complementary DNA</subject><subject>Crop diseases</subject><subject>disease resistance</subject><subject>DNA Primers</subject><subject>DNA, Complementary - genetics</subject><subject>downy mildew</subject><subject>genes</subject><subject>genetic markers</subject><subject>Genetic Markers - genetics</subject><subject>genetic resistance</subject><subject>Genetics</subject><subject>Helianthus - genetics</subject><subject>Helianthus annuus</subject><subject>Immunity, Innate - genetics</subject><subject>loci</subject><subject>marker-assisted selection</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Amplification Techniques</subject><subject>nucleotide sequences</subject><subject>Oomycetes</subject><subject>plant breeding</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - microbiology</subject><subject>plant pathogenic fungi</subject><subject>Plasmopara halstedii</subject><subject>Polymerase Chain Reaction</subject><subject>resistance gene analogs</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Tagged Sites</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkc9u1DAQxi0EokvhAbiAxYETbsd2HDtHCH-KtILVlp4tkx2zKU4c7ATEw_CuuMpKSBxGI838vm8sf4Q85XDBAfRlBuBCMICK8ZpLBvfIhldSMCEqcZ9sygKY0kqckUc53wKAUCAfkjOuilyB2pA_b_EnhjgNOM40erpr93Rw6TumTH1MdD4i3QV1uQuGhtgt6zRh7vPsxg7pHMve5SFOLjl6dCHPeOh72o80L6MP8RemV_QKQ-_G-Vj0bhyX0rYX1Kc40C4OU8AZaduyT2-u2Xa_pxl_LFjM82PywBdHfHLq5-Tm_bsv7RXbfv7wsX29ZZ0wMDPvQHJplKgcmkZx4VQtlUDtTdVprf2hQjh447Xm3nNQjYK6c6iqBmrhO3lOXq6-U4rldJ7t0OcOQ3AjxiVbLUBUpqkL-OI_8DYuaSxvs4Ybo-piXCC-Ql2KOSf0dkp9-dTfloO9C86uwdmSj70LzkLRPDsZL18HPPxTnJIqwPMV8C5a9y312d5cC-ASoKlKNfIva2GcIA</recordid><startdate>20040601</startdate><enddate>20040601</enddate><creator>Radwan, O</creator><creator>Bouzidi, M.F</creator><creator>Nicolas, P</creator><creator>Mouzeyar, S</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20040601</creationdate><title>Development of PCR markers for the Pl5/Pl8 locus for resistance to Plasmopara halstedii in sunflower, Helianthus annuus L. from complete CC-NBS-LRR sequences</title><author>Radwan, O ; Bouzidi, M.F ; Nicolas, P ; Mouzeyar, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c280t-fa03138524ae89512a56352e7f84c777fd4e0df8f771ff1059506cae549062fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>artificial selection</topic><topic>Base Sequence</topic><topic>Blotting, Southern</topic><topic>Chromosome Mapping</topic><topic>complementary DNA</topic><topic>Crop diseases</topic><topic>disease resistance</topic><topic>DNA Primers</topic><topic>DNA, Complementary - genetics</topic><topic>downy mildew</topic><topic>genes</topic><topic>genetic markers</topic><topic>Genetic Markers - genetics</topic><topic>genetic resistance</topic><topic>Genetics</topic><topic>Helianthus - genetics</topic><topic>Helianthus annuus</topic><topic>Immunity, Innate - genetics</topic><topic>loci</topic><topic>marker-assisted selection</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Amplification Techniques</topic><topic>nucleotide sequences</topic><topic>Oomycetes</topic><topic>plant breeding</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - microbiology</topic><topic>plant pathogenic fungi</topic><topic>Plasmopara halstedii</topic><topic>Polymerase Chain Reaction</topic><topic>resistance gene analogs</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Tagged Sites</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Radwan, O</creatorcontrib><creatorcontrib>Bouzidi, M.F</creatorcontrib><creatorcontrib>Nicolas, P</creatorcontrib><creatorcontrib>Mouzeyar, S</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Theoretical and applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Radwan, O</au><au>Bouzidi, M.F</au><au>Nicolas, P</au><au>Mouzeyar, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of PCR markers for the Pl5/Pl8 locus for resistance to Plasmopara halstedii in sunflower, Helianthus annuus L. from complete CC-NBS-LRR sequences</atitle><jtitle>Theoretical and applied genetics</jtitle><addtitle>Theor Appl Genet</addtitle><date>2004-06-01</date><risdate>2004</risdate><volume>109</volume><issue>1</issue><spage>176</spage><epage>185</epage><pages>176-185</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><abstract>Sunflower downy mildew, caused by Plasmopara halstedii, is one of the major diseases of this crop. Development of elite sunflower lines resistant to different races of this oomycete seems to be the most efficient method to limit downy mildew damage. At least two different gene clusters conferring resistance to different races of P. halstedii have been described. In this work we report the cloning and mapping of two full-length resistance gene analogs (RGA) belonging to the CC-NBC-LRR class of plant resistance genes. The two sequences were then used to develop 14 sequence tagged sites (STS) within the Pl5/Pl8 locus conferring resistance to a wide range of P. halstedii races. These STSs will be useful in marker-assisted selection programs.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>15007505</pmid><doi>10.1007/s00122-004-1613-0</doi><tpages>10</tpages></addata></record> |
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| ispartof | Theoretical and applied genetics, 2004-06, Vol.109 (1), p.176-185 |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Amino Acid Sequence amino acid sequences artificial selection Base Sequence Blotting, Southern Chromosome Mapping complementary DNA Crop diseases disease resistance DNA Primers DNA, Complementary - genetics downy mildew genes genetic markers Genetic Markers - genetics genetic resistance Genetics Helianthus - genetics Helianthus annuus Immunity, Innate - genetics loci marker-assisted selection Molecular Sequence Data Nucleic Acid Amplification Techniques nucleotide sequences Oomycetes plant breeding Plant Diseases - genetics Plant Diseases - microbiology plant pathogenic fungi Plasmopara halstedii Polymerase Chain Reaction resistance gene analogs Sequence Alignment Sequence Analysis, DNA Sequence Tagged Sites |
| title | Development of PCR markers for the Pl5/Pl8 locus for resistance to Plasmopara halstedii in sunflower, Helianthus annuus L. from complete CC-NBS-LRR sequences |
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