Development of PCR markers for the Pl5/Pl8 locus for resistance to Plasmopara halstedii in sunflower, Helianthus annuus L. from complete CC-NBS-LRR sequences

Sunflower downy mildew, caused by Plasmopara halstedii, is one of the major diseases of this crop. Development of elite sunflower lines resistant to different races of this oomycete seems to be the most efficient method to limit downy mildew damage. At least two different gene clusters conferring re...

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Veröffentlicht in:	Theoretical and applied genetics 2004-06, Vol.109 (1), p.176-185
Hauptverfasser:	Radwan, O, Bouzidi, M.F, Nicolas, P, Mouzeyar, S
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences artificial selection Base Sequence Blotting, Southern Chromosome Mapping complementary DNA Crop diseases disease resistance DNA Primers DNA, Complementary - genetics downy mildew genes genetic markers Genetic Markers - genetics genetic resistance Genetics Helianthus - genetics Helianthus annuus Immunity, Innate - genetics loci marker-assisted selection Molecular Sequence Data Nucleic Acid Amplification Techniques nucleotide sequences Oomycetes plant breeding Plant Diseases - genetics Plant Diseases - microbiology plant pathogenic fungi Plasmopara halstedii Polymerase Chain Reaction resistance gene analogs Sequence Alignment Sequence Analysis, DNA Sequence Tagged Sites
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Zusammenfassung:	Sunflower downy mildew, caused by Plasmopara halstedii, is one of the major diseases of this crop. Development of elite sunflower lines resistant to different races of this oomycete seems to be the most efficient method to limit downy mildew damage. At least two different gene clusters conferring resistance to different races of P. halstedii have been described. In this work we report the cloning and mapping of two full-length resistance gene analogs (RGA) belonging to the CC-NBC-LRR class of plant resistance genes. The two sequences were then used to develop 14 sequence tagged sites (STS) within the Pl5/Pl8 locus conferring resistance to a wide range of P. halstedii races. These STSs will be useful in marker-assisted selection programs.
ISSN:	0040-5752 1432-2242
DOI:	10.1007/s00122-004-1613-0