Inhibition of nonviral cationic liposome-mediated gene transfer into primary human respiratory cells by interferon-γ
The effect of interferon (IFN) gamma on cationic liposome-mediated gene transfer into primary respiratory epithelial cells was investigated. Treatment of primary respiratory epithelial cells with IFN-gamma resulted in a dose-dependent increase in the intermediate filament cytokeratin 13 and a decrea...
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| Veröffentlicht in: | Journal of molecular medicine (Berlin, Germany) Germany), 2002-08, Vol.80 (8), p.499-506 |
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| creator | SERSALE, Giovanna CARPANI, Daniela CASOTTI, Valeria LIVRAGHI, Alessandra CARRABINO, Salvatore DI CICCO, Maurizio ASSAEL, Baroukh M GIUNTA, Annamaria CONESE, Massimo |
| description | The effect of interferon (IFN) gamma on cationic liposome-mediated gene transfer into primary respiratory epithelial cells was investigated. Treatment of primary respiratory epithelial cells with IFN-gamma resulted in a dose-dependent increase in the intermediate filament cytokeratin 13 and a decrease in cellular proliferation, indicating that respiratory cells underwent squamous differentiation. IFN-gamma pretreatment resulted in a dramatic inhibition of transfection efficiency mediated by a cationic liposome (DOTAP). Incubation of squamous nasal cells with DOTAP/DNA complexes for various periods at 4 degrees C and evaluation of luciferase levels suggested that IFN-gamma pretreatment inhibits complex binding to the cells. In primary nasal and bronchial cells cytofluorimetric analysis demonstrated that IFN-gamma reduces binding of FITC-labeled complexes. The data indicate that differentiation of respiratory epithelial cells to a squamous phenotype, which may occur in chronic respiratory diseases such as cystic fibrosis, induces a refractory condition to gene transfer by nonviral cationic liposomes. |
| doi_str_mv | 10.1007/s00109-002-0352-4 |
| format | Article |
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Treatment of primary respiratory epithelial cells with IFN-gamma resulted in a dose-dependent increase in the intermediate filament cytokeratin 13 and a decrease in cellular proliferation, indicating that respiratory cells underwent squamous differentiation. IFN-gamma pretreatment resulted in a dramatic inhibition of transfection efficiency mediated by a cationic liposome (DOTAP). Incubation of squamous nasal cells with DOTAP/DNA complexes for various periods at 4 degrees C and evaluation of luciferase levels suggested that IFN-gamma pretreatment inhibits complex binding to the cells. In primary nasal and bronchial cells cytofluorimetric analysis demonstrated that IFN-gamma reduces binding of FITC-labeled complexes. The data indicate that differentiation of respiratory epithelial cells to a squamous phenotype, which may occur in chronic respiratory diseases such as cystic fibrosis, induces a refractory condition to gene transfer by nonviral cationic liposomes.</description><identifier>ISSN: 0946-2716</identifier><identifier>EISSN: 1432-1440</identifier><identifier>DOI: 10.1007/s00109-002-0352-4</identifier><identifier>PMID: 12185450</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Biological and medical sciences ; Biotechnology ; Cation Exchange Resins ; Cell Line, Transformed ; Cells, Cultured ; Cytokines - genetics ; Epithelial Cells - drug effects ; Epithelial Cells - physiology ; Fundamental and applied biological sciences. Psychology ; Gene transfer ; Gene Transfer Techniques ; Genetic engineering ; Genetic technics ; Genetic Vectors - drug effects ; Humans ; Interferon-gamma - metabolism ; Interferon-gamma - pharmacology ; Liposomes ; Luciferases - metabolism ; Methods. Procedures. Technologies ; Respiratory System - cytology ; Synthetic digonucleotides and genes. Sequencing ; Transfection</subject><ispartof>Journal of molecular medicine (Berlin, Germany), 2002-08, Vol.80 (8), p.499-506</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c327t-f95ddd5435be3bf5208e1dba286fe5ee1895604c8d3370879c774cb8fe9a4a1a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13856483$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12185450$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SERSALE, Giovanna</creatorcontrib><creatorcontrib>CARPANI, Daniela</creatorcontrib><creatorcontrib>CASOTTI, Valeria</creatorcontrib><creatorcontrib>LIVRAGHI, Alessandra</creatorcontrib><creatorcontrib>CARRABINO, Salvatore</creatorcontrib><creatorcontrib>DI CICCO, Maurizio</creatorcontrib><creatorcontrib>ASSAEL, Baroukh M</creatorcontrib><creatorcontrib>GIUNTA, Annamaria</creatorcontrib><creatorcontrib>CONESE, Massimo</creatorcontrib><title>Inhibition of nonviral cationic liposome-mediated gene transfer into primary human respiratory cells by interferon-γ</title><title>Journal of molecular medicine (Berlin, Germany)</title><addtitle>J Mol Med (Berl)</addtitle><description>The effect of interferon (IFN) gamma on cationic liposome-mediated gene transfer into primary respiratory epithelial cells was investigated. Treatment of primary respiratory epithelial cells with IFN-gamma resulted in a dose-dependent increase in the intermediate filament cytokeratin 13 and a decrease in cellular proliferation, indicating that respiratory cells underwent squamous differentiation. IFN-gamma pretreatment resulted in a dramatic inhibition of transfection efficiency mediated by a cationic liposome (DOTAP). Incubation of squamous nasal cells with DOTAP/DNA complexes for various periods at 4 degrees C and evaluation of luciferase levels suggested that IFN-gamma pretreatment inhibits complex binding to the cells. In primary nasal and bronchial cells cytofluorimetric analysis demonstrated that IFN-gamma reduces binding of FITC-labeled complexes. The data indicate that differentiation of respiratory epithelial cells to a squamous phenotype, which may occur in chronic respiratory diseases such as cystic fibrosis, induces a refractory condition to gene transfer by nonviral cationic liposomes.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cation Exchange Resins</subject><subject>Cell Line, Transformed</subject><subject>Cells, Cultured</subject><subject>Cytokines - genetics</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene transfer</subject><subject>Gene Transfer Techniques</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic Vectors - drug effects</subject><subject>Humans</subject><subject>Interferon-gamma - metabolism</subject><subject>Interferon-gamma - pharmacology</subject><subject>Liposomes</subject><subject>Luciferases - metabolism</subject><subject>Methods. Procedures. Technologies</subject><subject>Respiratory System - cytology</subject><subject>Synthetic digonucleotides and genes. Sequencing</subject><subject>Transfection</subject><issn>0946-2716</issn><issn>1432-1440</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1q3TAQRkVpaW7SPkA2RZt2p1S_lrwsIWkCgWzatZDlUaNgS7eSXchz9T36TJW5hqwGhvN9zByELhm9YpTqr5VSRntCKSdUKE7kG3RgUnDCpKRv0YH2siNcs-4Mndf63GitevkenTHOjJKKHtB6n57iEJeYE84Bp5z-xOIm7N22ih5P8ZhrnoHMMEa3wIh_QQK8FJdqgIJjWjI-lji78oKf1tklXKAeW8mS28bDNFU8vGwclBbIifz7-wG9C26q8HGfF-jn7c2P6zvy8Pj9_vrbA_GC64WEXo3jqKRQA4ghKE4NsHFw3HQBFAAzveqo9GYUQlOje6-19IMJ0DvpmBMX6Mup91jy7xXqYudYt5NcgrxWq3nTaDraQHYCfcm1Fgh2f8kyajfX9uTaNtd2c21ly3zay9ehyXlN7HIb8HkHXPVuCk2Zj_WVE0Z10gjxH91eimo</recordid><startdate>20020801</startdate><enddate>20020801</enddate><creator>SERSALE, Giovanna</creator><creator>CARPANI, Daniela</creator><creator>CASOTTI, Valeria</creator><creator>LIVRAGHI, Alessandra</creator><creator>CARRABINO, Salvatore</creator><creator>DI CICCO, Maurizio</creator><creator>ASSAEL, Baroukh M</creator><creator>GIUNTA, Annamaria</creator><creator>CONESE, Massimo</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020801</creationdate><title>Inhibition of nonviral cationic liposome-mediated gene transfer into primary human respiratory cells by interferon-γ</title><author>SERSALE, Giovanna ; CARPANI, Daniela ; CASOTTI, Valeria ; LIVRAGHI, Alessandra ; CARRABINO, Salvatore ; DI CICCO, Maurizio ; ASSAEL, Baroukh M ; GIUNTA, Annamaria ; CONESE, Massimo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c327t-f95ddd5435be3bf5208e1dba286fe5ee1895604c8d3370879c774cb8fe9a4a1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cation Exchange Resins</topic><topic>Cell Line, Transformed</topic><topic>Cells, Cultured</topic><topic>Cytokines - genetics</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene transfer</topic><topic>Gene Transfer Techniques</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic Vectors - drug effects</topic><topic>Humans</topic><topic>Interferon-gamma - metabolism</topic><topic>Interferon-gamma - pharmacology</topic><topic>Liposomes</topic><topic>Luciferases - metabolism</topic><topic>Methods. Procedures. Technologies</topic><topic>Respiratory System - cytology</topic><topic>Synthetic digonucleotides and genes. Sequencing</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SERSALE, Giovanna</creatorcontrib><creatorcontrib>CARPANI, Daniela</creatorcontrib><creatorcontrib>CASOTTI, Valeria</creatorcontrib><creatorcontrib>LIVRAGHI, Alessandra</creatorcontrib><creatorcontrib>CARRABINO, Salvatore</creatorcontrib><creatorcontrib>DI CICCO, Maurizio</creatorcontrib><creatorcontrib>ASSAEL, Baroukh M</creatorcontrib><creatorcontrib>GIUNTA, Annamaria</creatorcontrib><creatorcontrib>CONESE, Massimo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular medicine (Berlin, Germany)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SERSALE, Giovanna</au><au>CARPANI, Daniela</au><au>CASOTTI, Valeria</au><au>LIVRAGHI, Alessandra</au><au>CARRABINO, Salvatore</au><au>DI CICCO, Maurizio</au><au>ASSAEL, Baroukh M</au><au>GIUNTA, Annamaria</au><au>CONESE, Massimo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of nonviral cationic liposome-mediated gene transfer into primary human respiratory cells by interferon-γ</atitle><jtitle>Journal of molecular medicine (Berlin, Germany)</jtitle><addtitle>J Mol Med (Berl)</addtitle><date>2002-08-01</date><risdate>2002</risdate><volume>80</volume><issue>8</issue><spage>499</spage><epage>506</epage><pages>499-506</pages><issn>0946-2716</issn><eissn>1432-1440</eissn><abstract>The effect of interferon (IFN) gamma on cationic liposome-mediated gene transfer into primary respiratory epithelial cells was investigated. Treatment of primary respiratory epithelial cells with IFN-gamma resulted in a dose-dependent increase in the intermediate filament cytokeratin 13 and a decrease in cellular proliferation, indicating that respiratory cells underwent squamous differentiation. IFN-gamma pretreatment resulted in a dramatic inhibition of transfection efficiency mediated by a cationic liposome (DOTAP). Incubation of squamous nasal cells with DOTAP/DNA complexes for various periods at 4 degrees C and evaluation of luciferase levels suggested that IFN-gamma pretreatment inhibits complex binding to the cells. In primary nasal and bronchial cells cytofluorimetric analysis demonstrated that IFN-gamma reduces binding of FITC-labeled complexes. 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| subjects | Biological and medical sciences Biotechnology Cation Exchange Resins Cell Line, Transformed Cells, Cultured Cytokines - genetics Epithelial Cells - drug effects Epithelial Cells - physiology Fundamental and applied biological sciences. Psychology Gene transfer Gene Transfer Techniques Genetic engineering Genetic technics Genetic Vectors - drug effects Humans Interferon-gamma - metabolism Interferon-gamma - pharmacology Liposomes Luciferases - metabolism Methods. Procedures. Technologies Respiratory System - cytology Synthetic digonucleotides and genes. Sequencing Transfection |
| title | Inhibition of nonviral cationic liposome-mediated gene transfer into primary human respiratory cells by interferon-γ |
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