Using FM1-43 to study neuropeptide granule dynamics and exocytosis
In the study of neuropeptide secretion and membrane trafficking, the fluorescent dye FM1-43 provides the ability to label selectively those structures that are undergoing exocytosis and endocytosis in living cells in real time. This review describes the unique properties of the FM dyes that make the...
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| Veröffentlicht in: | Methods (San Diego, Calif.) Calif.), 2004-08, Vol.33 (4), p.287-294 |
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| container_title | Methods (San Diego, Calif.) |
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| creator | Brumback, Audrey C. Lieber, Janet L. Angleson, Joseph K. Betz, William J. |
| description | In the study of neuropeptide secretion and membrane trafficking, the fluorescent dye FM1-43 provides the ability to label selectively those structures that are undergoing exocytosis and endocytosis in living cells in real time. This review describes the unique properties of the FM dyes that make them ideal for studying neuropeptide granule dynamics and discusses various techniques that take advantage of FM dyes. |
| doi_str_mv | 10.1016/j.ymeth.2004.01.002 |
| format | Article |
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| ispartof | Methods (San Diego, Calif.), 2004-08, Vol.33 (4), p.287-294 |
| issn | 1046-2023 1095-9130 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Cytoplasmic Granules - secretion Exocytosis - physiology Fluorescent Dyes Genes, Reporter Genetic Techniques Immunohistochemistry - methods Neuropeptides - secretion Pyridinium Compounds Quaternary Ammonium Compounds |
| title | Using FM1-43 to study neuropeptide granule dynamics and exocytosis |
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