Using FM1-43 to study neuropeptide granule dynamics and exocytosis

Using FM1-43 to study neuropeptide granule dynamics and exocytosis

In the study of neuropeptide secretion and membrane trafficking, the fluorescent dye FM1-43 provides the ability to label selectively those structures that are undergoing exocytosis and endocytosis in living cells in real time. This review describes the unique properties of the FM dyes that make the...

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Veröffentlicht in:Methods (San Diego, Calif.) Calif.), 2004-08, Vol.33 (4), p.287-294
Hauptverfasser: Brumback, Audrey C., Lieber, Janet L., Angleson, Joseph K., Betz, William J.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cytoplasmic Granules - secretion
Exocytosis - physiology
Fluorescent Dyes
Genes, Reporter
Genetic Techniques
Immunohistochemistry - methods
Neuropeptides - secretion
Pyridinium Compounds
Quaternary Ammonium Compounds
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Zusammenfassung:In the study of neuropeptide secretion and membrane trafficking, the fluorescent dye FM1-43 provides the ability to label selectively those structures that are undergoing exocytosis and endocytosis in living cells in real time. This review describes the unique properties of the FM dyes that make them ideal for studying neuropeptide granule dynamics and discusses various techniques that take advantage of FM dyes.
ISSN:1046-2023
1095-9130
DOI:10.1016/j.ymeth.2004.01.002