Simple method for plant virus detection: effect of antibody immobilization technique

The possibility has been demonstrated for applying a surface plasmon resonance for detecting plant viruses in real samples. An optimal mode for antiviral immunoglobulin immobilization on sensor surfaces is described. Out of three proposed techniques for sensor surface treatment, namely, unmodified g...

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Veröffentlicht in:	Journal of virological methods 2002-08, Vol.105 (1), p.141-146
Hauptverfasser:	Boltovets, P.M, Boyko, V.R, Kostikov, I.Yu, Dyachenko, N.S, Snopok, B.A, Shirshov, Yu.M
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Viral - metabolism Bacteriology Biological and medical sciences Chlorophyta - virology Fundamental and applied biological sciences. Psychology Fundamental immunology Gold - metabolism Microbiology Molecular immunology Oriented protein immobilization Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Plant Diseases - virology Staphylococcal Protein A - metabolism Surface plasmon resonance Surface Plasmon Resonance - methods Surface Properties Techniques Thiocyanates - metabolism TMV Tobacco Mosaic Virus - isolation & purification Tobacco Mosaic Virus - metabolism
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Beschreibung
Zusammenfassung:	The possibility has been demonstrated for applying a surface plasmon resonance for detecting plant viruses in real samples. An optimal mode for antiviral immunoglobulin immobilization on sensor surfaces is described. Out of three proposed techniques for sensor surface treatment, namely, unmodified gold surface, gold surface treated with (a) thiocyanate and (b) thiocyanate and protein A ( Staphylococcus aureus), the latter was chosen as most suited for retention of the formed native immunoglobulin layer.
ISSN:	0166-0934 1879-0984
DOI:	10.1016/S0166-0934(02)00098-8