Ultrasensitive ATP Detection Using Firefly Luciferase Entrapped in Sugar-Modified Sol−Gel-Derived Silica

Ultrasensitive ATP Detection Using Firefly Luciferase Entrapped in Sugar-Modified Sol−Gel-Derived Silica

Firefly luciferase (FL) was entrapped in sol−gel-derived silica containing precursors based on covalent linkage of d-gluconolactone or d-maltonolactone to (aminopropyl)triethoxysilane to form N-(3-triethoxysilylpropyl)gluconamide or N-(3-triethoxysilylpropyl)maltonamide. The enzyme was active and st...

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Veröffentlicht in:Journal of the American Chemical Society 2004-06, Vol.126 (22), p.6878-6879
Hauptverfasser: Cruz-Aguado, Jorge A, Chen, Yang, Zhang, Zheng, Elowe, Nadine H, Brook, Michael A, Brennan, John D
Format: Artikel
Sprache:eng
Schlagworte:
Adenosine Triphosphate - analysis
Animals
Biological and medical sciences
Biosensors
Biotechnology
Carbohydrates - chemistry
Catalysis
Coleoptera - enzymology
Fundamental and applied biological sciences. Psychology
Gels - chemistry
Luciferases - metabolism
Methods. Procedures. Technologies
Reproducibility of Results
Sensitivity and Specificity
Silicon Dioxide - chemistry
Spectrometry, Fluorescence
Various methods and equipments
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Zusammenfassung:Firefly luciferase (FL) was entrapped in sol−gel-derived silica containing precursors based on covalent linkage of d-gluconolactone or d-maltonolactone to (aminopropyl)triethoxysilane to form N-(3-triethoxysilylpropyl)gluconamide or N-(3-triethoxysilylpropyl)maltonamide. The enzyme was active and stable in this material and showed catalytic constants close to those in solution. As little as 20 amol ATP could be detected with the entrapped FL, and the entrapped enzyme could be used over several cycles.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja0490424