Detecting single molecules launched from liquid surfaces in a time-of-flight mass spectrometer using ultraviolet and infrared lasers

The launch of molecules from liquid surfaces in a time of flight mass spectrometer has been investigated using different sample preparation techniques, and by exposing the liquid samples to two different laser wavelengths, 337 nm from a N2 ultraviolet laser and 10.6 νm from a CO2 infrared laser. The...

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Veröffentlicht in:Proteomics (Weinheim) 2002-04, Vol.2 (4), p.436-440
Hauptverfasser: Twerenbold, Damian, Netuschill, Alexandre, de Rooij, Nico, Luginbuhl, Phillipe, Gerber, Daniel, Gritti, Dominique, Gonin, Yuan, Rossel, Frédéric, Vuilleumier, Jean-Luc
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Sprache:eng
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Zusammenfassung:The launch of molecules from liquid surfaces in a time of flight mass spectrometer has been investigated using different sample preparation techniques, and by exposing the liquid samples to two different laser wavelengths, 337 nm from a N2 ultraviolet laser and 10.6 νm from a CO2 infrared laser. The molecules were detected with cryodetectors measuring the energy of the individual molecules. We present insulin and lysozyme results from samples introduced into the vacuum through a micromachined silicon injector, and from samples consisting of a glycerol droplet deposited directly on the sample holder at the high voltage stage of the ion optics.
ISSN:1615-9853
1615-9861
DOI:10.1002/1615-9861(200204)2:4<436::AID-PROT436>3.0.CO;2-I