Characterization of human CYP1A1/1A2 induction by DNA microarray and α-naphthoflavone

Characterization of human CYP1A1/1A2 induction by DNA microarray and α-naphthoflavone

DNA microarrays and real time PCR were used to analyze the mechanism of gene induction by CYP1A1 inducers, β-naphthoflavone, and omeprazole, in the human hepatocellular carcinoma HepG2 cells. Reproducible and significant inductions were observed in a limited number of genes including CYP1A1 and CYP1...

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Veröffentlicht in:Biochemical and biophysical research communications 2002-08, Vol.296 (1), p.172-177
Hauptverfasser: Ishida, Seiichi, Jinno, Hideto, Tanaka-Kagawa, Toshiko, Ando, Masanori, Ohno, Yasuo, Ozawa, Shogo, Sawada, Jun-ichi
Format: Artikel
Sprache:eng
Schlagworte:
Antigens, Differentiation - metabolism
Base Sequence
Benzoflavones - metabolism
Benzoflavones - pharmacology
Cell Line
CYP1A1
CYP1A2
Cytochrome P-450 CYP1A1 - genetics
Cytochrome P-450 CYP1A2 - genetics
DNA microarray
DNA Primers
Expression profiling
Galectin 3
Gene Expression Regulation, Enzymologic - drug effects
HepG2
Humans
Oligonucleotide Array Sequence Analysis
Omeprazole
Omeprazole - metabolism
Omeprazole - pharmacology
Plasminogen Activator Inhibitor 1 - metabolism
Protein tyrosine kinase
Protein-Tyrosine Kinases - metabolism
Transcriptional Activation
α-Naphthoflavone
β-Naphthoflavone
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Zusammenfassung:DNA microarrays and real time PCR were used to analyze the mechanism of gene induction by CYP1A1 inducers, β-naphthoflavone, and omeprazole, in the human hepatocellular carcinoma HepG2 cells. Reproducible and significant inductions were observed in a limited number of genes including CYP1A1 and CYP1A2. Genes induced by omeprazole included several protein tyrosine kinase targets. This result confirmed that omeprazole could modulate gene expressions through protein tyrosine kinase-mediated pathway. Induction ratios were considerably different from CYP1A1 and CYP1A2 (>10-fold) to other induced genes (
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(02)00856-2