High glucose-induced replicative senescence: point of no return and effect of telomerase

High glucose-induced replicative senescence: point of no return and effect of telomerase

Primary human cells enter senescence after a characteristic number of population doublings (PDs). In the current study, human skin fibroblasts were propagated in culture under 5.5 mM glucose (normoglycemia); addition of 16.5 mM d-glucose to a concentration of 22 mM (hyperglycemia); and addition of 1...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biochemical and biophysical research communications 2002-08, Vol.296 (1), p.93-101
Hauptverfasser: Blazer, Shraga, Khankin, Eli, Segev, Yardena, Ofir, Rachel, Yalon-Hacohen, Michal, Kra-Oz, Zipora, Gottfried, Yossi, Larisch, Sarit, Skorecki, Karl L
Format: Artikel
Sprache:eng
Schlagworte:
Apoptosis
Cell Division - drug effects
Cellular Senescence - drug effects
Culture Media
Diabetes
Fibroblasts - cytology
Fibroblasts - drug effects
Glucose - pharmacology
Humans
Hyperglycemia
Hyperosmolality
Replicative arrest
Senescence
Skin - cytology
Skin - drug effects
Skin fibroblasts
Telomerase
Telomerase - physiology
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Primary human cells enter senescence after a characteristic number of population doublings (PDs). In the current study, human skin fibroblasts were propagated in culture under 5.5 mM glucose (normoglycemia); addition of 16.5 mM d-glucose to a concentration of 22 mM (hyperglycemia); and addition of 16.5 mM l-glucose (osmotic control). Hyperglycemia induced premature replicative senescence after 44.42±1.5 PDs compared to 57.9±3.83 PDs under normoglycemia ( p
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(02)00818-5