Degradation of albumin by the renal proximal tubule cells and the subsequent fate of its fragments

Degradation of albumin by the renal proximal tubule cells and the subsequent fate of its fragments. In view of recent reports of large amounts of albumin fragments present in normal urine we investigated the mechanism of albumin handling by the proximal tubule. We injected 125I-albumin intravenously in rats and measured the excretion of intact and degraded 125I-albumin in the urine by trichloroacetic acid (TCA) precipitation. The excretion rate of intact 125I-albumin was compared to that obtained by routine radioimmunoassay (RIA). Human proximal tubular HK-2 cells were used to characterize the albumin receptor and study the degradation of albumin to peptides, establish their size by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration chromatography, and determine the direction in which the degradation products are removed from the cell. Following injection of 125I-albumin intravenously into rats we recovered large quantities of 125I-albumin fragments in urine and determined that 98% was in a highly degraded form and only 2% was intact. Only the intact albumin could be detected by RIA. We observed similar results in the urine of ex vivo kidneys perfused with 125I-albumin. We found that 125I-albumin was taken up by HK-2 cells via a receptor, degraded in the lysosomes and the peptides exocytosed to both the apical and basolateral sides of the cells. We conclude that normally the kidney degrades large amounts of albumin and that the degradation fragments appear in the urine. These findings are in sharp contrast with the established view that degraded albumin is completely reabsorbed into the blood stream.