Hypomorphic expression of Dkk1 in the doubleridge mouse: dose dependence and compensatory interactions with Lrp6

doubleridge is a transgene-induced mouse mutation displaying forelimb postaxial polysyndactyly. We have cloned the doubleridge transgene insertion site and demonstrate that doubleridge acts in cis from a distance of 150 kb to reduce the expression of dickkopf 1 ( Dkk1 ), the secreted Wnt antagonist....

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Veröffentlicht in:	Development (Cambridge) 2004-06, Vol.131 (11), p.2543-2552
Hauptverfasser:	MacDonald, Bryan T, Adamska, Maja, Meisler, Miriam H
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Chromosomes Cloning, Molecular DNA Transposable Elements Exons Fetal Death - genetics Gene Dosage Gene Expression Regulation, Developmental Gene Rearrangement Genetic Complementation Test Head - abnormalities Head - embryology Intercellular Signaling Peptides and Proteins LDL-Receptor Related Proteins Limb Deformities, Congenital - embryology Limb Deformities, Congenital - genetics Low Density Lipoprotein Receptor-Related Protein-5 Low Density Lipoprotein Receptor-Related Protein-6 Mice Mice, Mutant Strains Mice, Transgenic Molecular Sequence Data Proteins - genetics Proteins - metabolism Receptors, LDL - genetics Receptors, LDL - metabolism Signal Transduction Spine - abnormalities Spine - embryology Syndactyly - genetics
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Zusammenfassung:	doubleridge is a transgene-induced mouse mutation displaying forelimb postaxial polysyndactyly. We have cloned the doubleridge transgene insertion site and demonstrate that doubleridge acts in cis from a distance of 150 kb to reduce the expression of dickkopf 1 ( Dkk1 ), the secreted Wnt antagonist. Expression of Dkk1 from the doubleridge allele ranges from 35% of wild-type level in E7.0 head to
ISSN:	0950-1991 1477-9129
DOI:	10.1242/dev.01126