In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel
There is a clinical need for synthetic scaffolds that promote bone regeneration. A common problem encountered when using scaffolds in tissue engineering is the rapid formation of tissue on the outer edge of the scaffold whilst the tissue in the centre becomes necrotic. To address this, the scaffold...
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Veröffentlicht in: | Biomaterials 2004-11, Vol.25 (24), p.5507-5514 |
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creator | Rose, Felicity R. Cyster, Lesley A. Grant, David M. Scotchford, Colin A. Howdle, Steven M. Shakesheff, Kevin M. |
description | There is a clinical need for synthetic scaffolds that promote bone regeneration. A common problem encountered when using scaffolds in tissue engineering is the rapid formation of tissue on the outer edge of the scaffold whilst the tissue in the centre becomes necrotic. To address this, the scaffold design should improve nutrient and cell transfer to the scaffold centre. In this study, hydroxyapatite scaffolds with random, open porosity (average pore size of 282±11
μm, average interconnecting window size of 72±4
μm) were manufactured using a modified slip-casting methodology with a single aligned channel inserted into the centre. By varying the aligned channel diameter, a series of scaffolds with channel diameters ranging from 170 to 421
μm were produced. These scaffolds were seeded with human osteosarcoma (HOS TE85) cells and cultured for 8 days. Analysis of cell penetration into the aligned channels revealed that cell coverage increased with increasing channel diameter; from 22±3% in the 170
μm diameter channel to 38±6% coverage in the 421
μm channel. Cell penetration into the middle section of the 421
μm diameter channel (average cell area coverage 121×10
3±32×10
3
μm
2) was significantly greater than that observed within the 170
μm channel (average cell area coverage 26×10
3±6×10
3
μm
2). In addition, the data presented demonstrates that the minimum channel (or pore) diameter required for cell penetration into such scaffolds is approximately 80
μm. These results will direct the development of scaffolds with aligned macroarchitecture for tissue engineering bone. |
doi_str_mv | 10.1016/j.biomaterials.2004.01.012 |
format | Article |
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μm, average interconnecting window size of 72±4
μm) were manufactured using a modified slip-casting methodology with a single aligned channel inserted into the centre. By varying the aligned channel diameter, a series of scaffolds with channel diameters ranging from 170 to 421
μm were produced. These scaffolds were seeded with human osteosarcoma (HOS TE85) cells and cultured for 8 days. Analysis of cell penetration into the aligned channels revealed that cell coverage increased with increasing channel diameter; from 22±3% in the 170
μm diameter channel to 38±6% coverage in the 421
μm channel. Cell penetration into the middle section of the 421
μm diameter channel (average cell area coverage 121×10
3±32×10
3
μm
2) was significantly greater than that observed within the 170
μm channel (average cell area coverage 26×10
3±6×10
3
μm
2). In addition, the data presented demonstrates that the minimum channel (or pore) diameter required for cell penetration into such scaffolds is approximately 80
μm. These results will direct the development of scaffolds with aligned macroarchitecture for tissue engineering bone.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2004.01.012</identifier><identifier>PMID: 15142732</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Bone tissue engineering ; Cell Division ; Durapatite ; Hydroxyapatite ; Macroarchitecture ; Microscopy, Electron, Scanning ; Osteoblast ; Scaffold ; Tissue Engineering</subject><ispartof>Biomaterials, 2004-11, Vol.25 (24), p.5507-5514</ispartof><rights>2004 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-e9db1f97cbe8ecf82f0d15bb1790171aceef2305674e9e1a0899b82fc3fda3ea3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961204000134$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15142732$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rose, Felicity R.</creatorcontrib><creatorcontrib>Cyster, Lesley A.</creatorcontrib><creatorcontrib>Grant, David M.</creatorcontrib><creatorcontrib>Scotchford, Colin A.</creatorcontrib><creatorcontrib>Howdle, Steven M.</creatorcontrib><creatorcontrib>Shakesheff, Kevin M.</creatorcontrib><title>In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>There is a clinical need for synthetic scaffolds that promote bone regeneration. A common problem encountered when using scaffolds in tissue engineering is the rapid formation of tissue on the outer edge of the scaffold whilst the tissue in the centre becomes necrotic. To address this, the scaffold design should improve nutrient and cell transfer to the scaffold centre. In this study, hydroxyapatite scaffolds with random, open porosity (average pore size of 282±11
μm, average interconnecting window size of 72±4
μm) were manufactured using a modified slip-casting methodology with a single aligned channel inserted into the centre. By varying the aligned channel diameter, a series of scaffolds with channel diameters ranging from 170 to 421
μm were produced. These scaffolds were seeded with human osteosarcoma (HOS TE85) cells and cultured for 8 days. Analysis of cell penetration into the aligned channels revealed that cell coverage increased with increasing channel diameter; from 22±3% in the 170
μm diameter channel to 38±6% coverage in the 421
μm channel. Cell penetration into the middle section of the 421
μm diameter channel (average cell area coverage 121×10
3±32×10
3
μm
2) was significantly greater than that observed within the 170
μm channel (average cell area coverage 26×10
3±6×10
3
μm
2). In addition, the data presented demonstrates that the minimum channel (or pore) diameter required for cell penetration into such scaffolds is approximately 80
μm. These results will direct the development of scaffolds with aligned macroarchitecture for tissue engineering bone.</description><subject>Bone tissue engineering</subject><subject>Cell Division</subject><subject>Durapatite</subject><subject>Hydroxyapatite</subject><subject>Macroarchitecture</subject><subject>Microscopy, Electron, Scanning</subject><subject>Osteoblast</subject><subject>Scaffold</subject><subject>Tissue Engineering</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1vEzEQhi1ERUPLX0AWB24bPN7deM0Nla9KlXqhZ8trj4nDrr3YTiH_HkeJBDcqjTSy5nk9Hy8hb4CtgcHm3W49-jjrgsnrKa85Y92aQQ3-jKxgEEPTS9Y_JysGHW_kBvgleZnzjtU36_gLcgl9rYiWr8iP20AffUmR6pwx5xlDodFRg9NEFwxYki4-BupDiXSJKe4z3R5sir8PeqmlgjQb7VycbKa_fNlSXcWhyiaqJ_89oKVmq0PA6ZpcuDowvjrnK_Lw-dO3m6_N3f2X25sPd43pNqI0KO0ITgoz4oDGDdwxC_04gpAMBGiD6HjL-o3oUCJoNkg5Vsq0zuoWdXtF3p7-XVL8ucdc1OzzcSEdsI6vBEjORS__C_Kh5cBl9wSQy5azvoLvT6BJMeeETi3JzzodFDB1NE_t1L_mqaN5ikENXsWvz13244z2r_TsVgU-ngCs13v0mFQ2HoNB6xOaomz0T-nzB6bhtOw</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Rose, Felicity R.</creator><creator>Cyster, Lesley A.</creator><creator>Grant, David M.</creator><creator>Scotchford, Colin A.</creator><creator>Howdle, Steven M.</creator><creator>Shakesheff, Kevin M.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QQ</scope><scope>8FD</scope><scope>JG9</scope><scope>F28</scope><scope>FR3</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel</title><author>Rose, Felicity R. ; Cyster, Lesley A. ; Grant, David M. ; Scotchford, Colin A. ; Howdle, Steven M. ; Shakesheff, Kevin M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-e9db1f97cbe8ecf82f0d15bb1790171aceef2305674e9e1a0899b82fc3fda3ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Bone tissue engineering</topic><topic>Cell Division</topic><topic>Durapatite</topic><topic>Hydroxyapatite</topic><topic>Macroarchitecture</topic><topic>Microscopy, Electron, Scanning</topic><topic>Osteoblast</topic><topic>Scaffold</topic><topic>Tissue Engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rose, Felicity R.</creatorcontrib><creatorcontrib>Cyster, Lesley A.</creatorcontrib><creatorcontrib>Grant, David M.</creatorcontrib><creatorcontrib>Scotchford, Colin A.</creatorcontrib><creatorcontrib>Howdle, Steven M.</creatorcontrib><creatorcontrib>Shakesheff, Kevin M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Ceramic Abstracts</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rose, Felicity R.</au><au>Cyster, Lesley A.</au><au>Grant, David M.</au><au>Scotchford, Colin A.</au><au>Howdle, Steven M.</au><au>Shakesheff, Kevin M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>25</volume><issue>24</issue><spage>5507</spage><epage>5514</epage><pages>5507-5514</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>There is a clinical need for synthetic scaffolds that promote bone regeneration. A common problem encountered when using scaffolds in tissue engineering is the rapid formation of tissue on the outer edge of the scaffold whilst the tissue in the centre becomes necrotic. To address this, the scaffold design should improve nutrient and cell transfer to the scaffold centre. In this study, hydroxyapatite scaffolds with random, open porosity (average pore size of 282±11
μm, average interconnecting window size of 72±4
μm) were manufactured using a modified slip-casting methodology with a single aligned channel inserted into the centre. By varying the aligned channel diameter, a series of scaffolds with channel diameters ranging from 170 to 421
μm were produced. These scaffolds were seeded with human osteosarcoma (HOS TE85) cells and cultured for 8 days. Analysis of cell penetration into the aligned channels revealed that cell coverage increased with increasing channel diameter; from 22±3% in the 170
μm diameter channel to 38±6% coverage in the 421
μm channel. Cell penetration into the middle section of the 421
μm diameter channel (average cell area coverage 121×10
3±32×10
3
μm
2) was significantly greater than that observed within the 170
μm channel (average cell area coverage 26×10
3±6×10
3
μm
2). In addition, the data presented demonstrates that the minimum channel (or pore) diameter required for cell penetration into such scaffolds is approximately 80
μm. These results will direct the development of scaffolds with aligned macroarchitecture for tissue engineering bone.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>15142732</pmid><doi>10.1016/j.biomaterials.2004.01.012</doi><tpages>8</tpages></addata></record> |
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subjects | Bone tissue engineering Cell Division Durapatite Hydroxyapatite Macroarchitecture Microscopy, Electron, Scanning Osteoblast Scaffold Tissue Engineering |
title | In vitro assessment of cell penetration into porous hydroxyapatite scaffolds with a central aligned channel |
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