Peptide Arrays for Highly Sensitive and Specific Antibody-Binding Fluorescence Assays

We report a novel generation of peptide arrays fabricated by site-specific ligation of glyoxylyl peptides onto glass slides covered by a semicarbazide sol−gel layer. These arrays allowed the highly sensitive and specific detection of antibodies in very small blood samples from infected individuals u...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Bioconjugate chemistry 2002-07, Vol.13 (4), p.713-720
Hauptverfasser: Melnyk, Oleg, Duburcq, Xavier, Olivier, Christophe, Urbès, Florence, Auriault, Claude, Gras-Masse, Hélène
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:We report a novel generation of peptide arrays fabricated by site-specific ligation of glyoxylyl peptides onto glass slides covered by a semicarbazide sol−gel layer. These arrays allowed the highly sensitive and specific detection of antibodies in very small blood samples from infected individuals using three model peptidic epitopes (HCV Core and NS4, EBV Capsid) in an immunofluorescence assay. Comparison with standard enzyme-linked immunosorbent assays (ELISAs) demonstrated a large gain in sensitivity and specificity. These unique properties, combined with the possibility to immobilize glycoproteins such as antibodies, offer the possibility to perform sandwich immunofluorescent assays in a highly parallel format.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc015584o