Oxidative damage to DNA, p53 gene expression and p53 protein level in the process of aging in rat brain

Levels of 8-oxo2′dG (HPLC), p53 mRNA (PCR) and p53 protein (Western Blot) were estimated in four structures of rat brain, including grey matter (GM) of cerebral cortex, cerebral white matter (WM), cerebellum (C) and medulla oblongata (MO) of control (3.0–3.5-month-old) rats, 12- and 24-month-old rat...

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Veröffentlicht in:	Respiratory physiology & neurobiology 2004-02, Vol.139 (3), p.227-236
Hauptverfasser:	Dorszewska, Jolanta, Adamczewska-Goncerzewicz, Zofia
Format:	Artikel
Sprache:	eng
Schlagworte:	Aging - physiology Aging, oxidative damage Animals Biochemistry and metabolism Biological and medical sciences Blotting, Western - methods Brain - anatomy & histology Brain - metabolism Brain Chemistry - physiology Central nervous system Chromatography, High Pressure Liquid - methods Deoxyadenosines - analysis DNA - metabolism DNA, oxidative damage Female Fundamental and applied biological sciences. Psychology Gene Expression - physiology Gene, expression, p53 Mammals, rat Oxidative Stress - physiology Polymerase Chain Reaction - methods Protein, p53 Rats Rats, Wistar RNA - metabolism Statistics, Nonparametric Tumor Suppressor Protein p53 - genetics Tumor Suppressor Protein p53 - metabolism Vertebrates: nervous system and sense organs
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Zusammenfassung:	Levels of 8-oxo2′dG (HPLC), p53 mRNA (PCR) and p53 protein (Western Blot) were estimated in four structures of rat brain, including grey matter (GM) of cerebral cortex, cerebral white matter (WM), cerebellum (C) and medulla oblongata (MO) of control (3.0–3.5-month-old) rats, 12- and 24-month-old rats. The level of oxidative DNA was statistically significantly higher in C of 24-month-old animals. Expression of p53 gene increased in C and also in the all other investigated brain parts, while the protein level of p53 was enhanced only in GM of 24-month-old rats. These data indicated that DNA oxidative damage and p53 gene expression increased significantly in aged brain. The higher expression of p53 gene in aged brain may suggest the activation of DNA repair processes.
ISSN:	1569-9048 1878-1519
DOI:	10.1016/j.resp.2003.10.005