Osteopontin Plays an Important Role in the Development of Medial Thickening and Neointimal Formation

Osteopontin (OPN) is a soluble secreted phosphoprotein that binds with high affinity to several integrins and it has been found at the site of atherosclerotic lesions. However, the role of OPN expression in vivo is still poorly understood. To investigate the physiological role of OPN in detail, we g...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Circulation research 2002-07, Vol.91 (1), p.77-82
Hauptverfasser:	Isoda, Kikuo, Nishikawa, Kenichirou, Kamezawa, Yashuhiro, Yoshida, Mikoto, Kusuhara, Masatoshi, Moroi, Masao, Tada, Norihiro, Ohsuzu, Fumitaka
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Aorta - metabolism Aorta - pathology Aorta - physiopathology Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels Cardiology. Vascular system Cell Movement - physiology Female Femoral Artery - injuries Gene Expression Immunohistochemistry Male Matrix Metalloproteinase 2 - metabolism Matrix Metalloproteinase 9 - metabolism Medical sciences Mice Mice, Inbred C57BL Mice, Inbred Strains Mice, Transgenic Muscle, Smooth, Vascular - cytology Osteopontin Proliferating Cell Nuclear Antigen - analysis RNA, Messenger - genetics RNA, Messenger - metabolism Sialoglycoproteins - genetics Sialoglycoproteins - physiology Tunica Intima - metabolism Tunica Intima - pathology Tunica Intima - physiopathology Tunica Media - metabolism Tunica Media - pathology Tunica Media - physiopathology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Osteopontin (OPN) is a soluble secreted phosphoprotein that binds with high affinity to several integrins and it has been found at the site of atherosclerotic lesions. However, the role of OPN expression in vivo is still poorly understood. To investigate the physiological role of OPN in detail, we generated transgenic mice (Tg) overexpressing the OPN gene under control of the cytomegalovirus enhancer/chicken β-actin promoter. We detected OPN mRNAs in almost all tissues of 3 lines of Tg mice by Northern blotting. The serum levels of OPN were significantly higher in Tg than in non-Tg mice (782±107 versus 182±44 ng/mL;P
ISSN:	0009-7330 1524-4571
DOI:	10.1161/01.RES.0000025268.10302.0C