Base pairing properties of 8-oxo-7,8-dihydroadenosine in cDNA synthesis by reverse transcriptases

Incorporation of nucleotides opposite 8-oxo-7,8-dihydroadenosine (8-oxoA) in oligonucleotides with dNTPs by three reverse transcriptases (AMV-, MMRV-, RAV2-RT) in cDNA synthesis was studied. Guanine as well as thymine was incorporated preferentially by all reverse transcriptases. In the melting temp...

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Veröffentlicht in:	Bioorganic & medicinal chemistry letters 2002-08, Vol.12 (15), p.1977-1980
Hauptverfasser:	Kim, Sang Kook, Kim, Ji Young, Baek, Ae Kyeong, Moon, Byung Jo
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Autoradiography Base Pairing Base Sequence Biological and medical sciences Carbohydrates. Nucleosides and nucleotides Chemistry Deoxyadenosines - chemistry Deoxyribonucleotides - chemistry DNA Primers - chemistry DNA, Complementary - chemical synthesis Dna, deoxyribonucleoproteins Exact sciences and technology Fundamental and applied biological sciences. Psychology Guanosine - analogs & derivatives Nucleic Acid Denaturation Nucleic acids Nucleosides, nucleotides and oligonucleotides Oligonucleotides - chemistry Organic chemistry Preparations and properties RNA - chemistry RNA-Directed DNA Polymerase - chemistry Temperature Templates, Genetic Thermodynamics Thymine - analogs & derivatives
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Zusammenfassung:	Incorporation of nucleotides opposite 8-oxo-7,8-dihydroadenosine (8-oxoA) in oligonucleotides with dNTPs by three reverse transcriptases (AMV-, MMRV-, RAV2-RT) in cDNA synthesis was studied. Guanine as well as thymine was incorporated preferentially by all reverse transcriptases. In the melting temperature experiment, 8-oxoA and 8-oxoA-Me formed base pairs with thymine and guanine with similar stabilities. Incorporation of nucleotides opposite 8-oxo-7,8-dihydroadenosine (8-oxoA) in oligonucleotides with dNTPs by three reverse transcriptases (AMV-, MMRV-, RAV2-RT) in cDNA synthesis was studied. Guanine as well as thymine was incorporated preferentially by all reverse transcriptases. In the melting temperature experiment, 8-oxoA and 8-oxoA-Me formed base pairs with thymine and guanine with similar stabilities.
ISSN:	0960-894X 1464-3405
DOI:	10.1016/S0960-894X(02)00297-4