Excitatory effects of ATP on rat dorsomedial hypothalamic neurons

We investigated P2X purinoceptors in rat dorsomedial hypothalamic (DMH) neurons using nystatin-perforated patch-clamp recordings and fura-2 microfluorometry. Adenosine triphosphate (ATP) concentration-dependently evoked an inward current and increased cytosolic Ca 2+ ([Ca] i). The rise in [Ca] i was...

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Veröffentlicht in:	Brain research 2004-05, Vol.1009 (1), p.234-237
Hauptverfasser:	Matsumoto, N., Sorimachi, M., Akaike, N.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - analogs & derivatives Adenosine Triphosphate - pharmacology Animals Animals, Newborn Biological and medical sciences Ca 2+ channel Cadmium - pharmacology Calcium - metabolism Calcium - pharmacology Calcium Channel Blockers - pharmacology Cells, Cultured Central nervous system Dorsomedial hypothalamic neuron Dose-Response Relationship, Drug Electrophysiology Fundamental and applied biological sciences. Psychology Fura-2 - pharmacokinetics Fura-2 microfluorometry Hypothalamus, Middle - cytology Membrane Potentials - drug effects Neurons - drug effects Neurons - physiology Nystatin-perforated patch-clamp P2X purinoceptor Patch-Clamp Techniques Rats Rats, Wistar Vertebrates: nervous system and sense organs
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Zusammenfassung:	We investigated P2X purinoceptors in rat dorsomedial hypothalamic (DMH) neurons using nystatin-perforated patch-clamp recordings and fura-2 microfluorometry. Adenosine triphosphate (ATP) concentration-dependently evoked an inward current and increased cytosolic Ca 2+ ([Ca] i). The rise in [Ca] i was dependent on external Ca 2+ and Na +, was blocked by Ca 2+ channel antagonists and had pharmacological properties consistent with P2X2 receptors. These results suggest that P2X receptor-mediated depolarization activates voltage-gated Ca 2+ channels, resulting in an increase in [Ca] i.
ISSN:	0006-8993 1872-6240
DOI:	10.1016/j.brainres.2004.03.001