Technical Evaluation of a New Immunoradiometric and a New Immunoluminometric Assay for Thyroglobulin

After removal of differentiated thyroid carcinoma (DTC), serum thyroglobulin (Tg) can indicate persistent or recurrent disease. We describe two novel two-step assays designed to measure low Tg concentrations. We evaluated prototypes of the new IRMA, DYNOtest Tg-pluS, and the new immunoluminometric a...

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Veröffentlicht in:	Clinical chemistry (Baltimore, Md.) Md.), 2002-07, Vol.48 (7), p.1077-1083
Hauptverfasser:	Morgenthaler, Nils G, Froehlich, Juergen, Rendl, Johann, Willnich, Marita, Alonso, Christine, Bergmann, Andreas, Reiners, Christoph
Format:	Artikel
Sprache:	eng
Schlagworte:	Autoantibodies - blood Biological and medical sciences Cross Reactions Humans Immunoassay - methods Immunoradiometric Assay Investigative techniques, diagnostic techniques (general aspects) Medical sciences Miscellaneous. Technology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Reproducibility of Results Sensitivity and Specificity Thyroglobulin - blood Thyroglobulin - immunology Thyroid Neoplasms - diagnosis Thyroiditis, Autoimmune - diagnosis Thyrotropin - blood
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Zusammenfassung:	After removal of differentiated thyroid carcinoma (DTC), serum thyroglobulin (Tg) can indicate persistent or recurrent disease. We describe two novel two-step assays designed to measure low Tg concentrations. We evaluated prototypes of the new IRMA, DYNOtest Tg-pluS, and the new immunoluminometric assay (ILMA), LUMItest) Tg-pluS. In the first step, a high-salt incubation buffer leads to dissociation of Tg-Tg antibody complexes in serum and is intended to reduce nonspecific interference and interference of potential Tg autoantibodies in the system. We studied recovery of human Tg (from thyroid glands) added to horse serum. We also studied 58 patients with DTC in whom Tg values under thyroid-stimulating hormone (TSH) suppression and TSH stimulation (without thyroxine) were available. The detection limits were 0.04 microg/L Tg for the IRMA and 0.02 microg/L for the ILMA. Intraassay imprecision (CV) was
ISSN:	0009-9147 1530-8561
DOI:	10.1093/clinchem/48.7.1077