Analysis of Sugar Epimers Using Mass Spectrometry: N-Acetyllactosamine-6,6′-Disulfate and the 2′-Epimer

Analysis of Sugar Epimers Using Mass Spectrometry: N-Acetyllactosamine-6,6′-Disulfate and the 2′-Epimer

Galβ1–4GlcNAc-6,6′-disulfate and 2′-epimer corresponding to Galβ1–4ManNAc-6,6′-disulfate were distinguished by mass spectrometry by utilizing fast atom bombardment (FAB), electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) methods. As for the steric information, neg...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:European journal of mass spectrometry (Chichester, England) England), 2004-01, Vol.10 (2), p.269-278
Hauptverfasser: Ohashi, Yoko, Itoh, Yoshiyuki, Kubota, Masayuki, Hamada, Kensaku, Ohashi, Mamoru, Hirano, Takashi, Niwa, Haruki
Format: Artikel
Sprache:eng
Schlagworte:
Amino Sugars - chemistry
Carbohydrate Conformation
Carbohydrate Sequence
Mass Spectrometry
Models, Molecular
Spectrometry, Mass, Electrospray Ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Galβ1–4GlcNAc-6,6′-disulfate and 2′-epimer corresponding to Galβ1–4ManNAc-6,6′-disulfate were distinguished by mass spectrometry by utilizing fast atom bombardment (FAB), electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) methods. As for the steric information, negative-ion ESI mass spectrometry/mass spectrometry (MS/MS) provides the most extensive data, but FAB MS/MS also reveals detailed structural information of interest in our case, where MALDI MS is not yet fully equipped with post-source decay.
ISSN:1469-0667
1751-6838
DOI:10.1255/ejms.629