Genetic vaccines protect against Sin Nombre hantavirus challenge in the deer mouse (Peromyscus maniculatus)
Center for Emerging Infectious Diseases, Departments of Pathology 1 , Medicine 2 and Biology and Molecular Genetics and Microbiology 3 , University of New Mexico School of Medicine, Albuquerque, NM 87131, USA Author for correspondence: Brian Hjelle (at Department of Pathology). Fax +1 505 272 9912....
Gespeichert in:
| Veröffentlicht in: | Journal of general virology 2002-07, Vol.83 (7), p.1745-1751 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1751 |
|---|---|
| container_issue | 7 |
| container_start_page | 1745 |
| container_title | Journal of general virology |
| container_volume | 83 |
| creator | Bharadwaj, Mausumi Mirowsky, Katy Ye, Chunyan Botten, Jason Masten, Barbara Yee, Joyce Lyons, C. Richard Hjelle, Brian |
| description | Center for Emerging Infectious Diseases, Departments of Pathology 1 , Medicine 2 and Biology and Molecular Genetics and Microbiology 3 , University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
Author for correspondence: Brian Hjelle (at Department of Pathology). Fax +1 505 272 9912. e-mail bhjelle{at}salud.unm.edu
We used a deer mouse ( Peromyscus maniculatus ) infection model to test the protective efficacy of genetic vaccine candidates for Sin Nombre (SN) virus that were known to provoke immunological responses in BALB/c mice (Bharadwaj et al ., Vaccine 17, 28362843, 1999 ). Protective epitopes were localized in each of four overlapping cDNA fragments that encoded portions of the SN virus G1 glycoprotein antigen; the nucleocapsid gene also was protective. The protective efficacy of glycoprotein gene fragments correlated with splenocyte proliferation in the presence of cognate antigen, but none induced neutralizing antibodies. Genetic vaccines against SN virus can protect outbred deer mice from infection even in the absence of a neutralizing antibody response. |
| doi_str_mv | 10.1099/0022-1317-83-7-1745 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71840815</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71840815</sourcerecordid><originalsourceid>FETCH-LOGICAL-c379t-ad1804f388625eaefef56eeb35506706e29071d6d436adb361024576c4e3eac03</originalsourceid><addsrcrecordid>eNpFkE1P3DAQhi0EgmXpL0BCPiE4pLXjr-SIECxIqFQqPVuOM9kYEgdsh4p_j1e7gtMc5pl3Zh6ETin5SUld_yKkLAvKqCoqVqiCKi720IJyKYoy9_fR4os4QscxPhNCORfqEB3RkihBar5ALyvwkJzF78Za5yHi1zAlsAmbtXE-JvzXefx7GpsAuDc-mXcX5ohtb4YB_BpwbqcecAsQ8DjNEfDFHwjT-BFt5kbjnZ0Hk-Z4eYIOOjNE-LGrS_Tv9ubp-q54eFzdX189FJapOhWmpRXhHasqWQow0EEnJEDDhCBSEQllTRRtZcuZNG3DJCVlfktaDgyMJWyJzre5-ZW3GWLSo4sWhsF4yAdqRStOKioyyLagDVOMATr9GtxowoemRG8c641BvTGoK6aV3jjOU2e7-LkZof2e2UnNwMUW6N26_-8C6DX40eUljZt09ved9Qk3gIZv</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71840815</pqid></control><display><type>article</type><title>Genetic vaccines protect against Sin Nombre hantavirus challenge in the deer mouse (Peromyscus maniculatus)</title><source>MEDLINE</source><source>Microbiology Society</source><source>Alma/SFX Local Collection</source><source>EZB Electronic Journals Library</source><creator>Bharadwaj, Mausumi ; Mirowsky, Katy ; Ye, Chunyan ; Botten, Jason ; Masten, Barbara ; Yee, Joyce ; Lyons, C. Richard ; Hjelle, Brian</creator><creatorcontrib>Bharadwaj, Mausumi ; Mirowsky, Katy ; Ye, Chunyan ; Botten, Jason ; Masten, Barbara ; Yee, Joyce ; Lyons, C. Richard ; Hjelle, Brian</creatorcontrib><description>Center for Emerging Infectious Diseases, Departments of Pathology 1 , Medicine 2 and Biology and Molecular Genetics and Microbiology 3 , University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
Author for correspondence: Brian Hjelle (at Department of Pathology). Fax +1 505 272 9912. e-mail bhjelle{at}salud.unm.edu
We used a deer mouse ( Peromyscus maniculatus ) infection model to test the protective efficacy of genetic vaccine candidates for Sin Nombre (SN) virus that were known to provoke immunological responses in BALB/c mice (Bharadwaj et al ., Vaccine 17, 28362843, 1999 ). Protective epitopes were localized in each of four overlapping cDNA fragments that encoded portions of the SN virus G1 glycoprotein antigen; the nucleocapsid gene also was protective. The protective efficacy of glycoprotein gene fragments correlated with splenocyte proliferation in the presence of cognate antigen, but none induced neutralizing antibodies. Genetic vaccines against SN virus can protect outbred deer mice from infection even in the absence of a neutralizing antibody response.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-83-7-1745</identifier><identifier>PMID: 12075094</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Animals ; Antibodies, Viral - blood ; DNA, Complementary ; Hantavirus Infections - immunology ; Hantavirus Infections - prevention & control ; Injections, Intramuscular ; Mice ; Nucleocapsid - genetics ; Nucleocapsid - immunology ; Peromyscus ; Sin Nombre virus - genetics ; Sin Nombre virus - immunology ; Spleen - cytology ; Spleen - immunology ; Vaccines, DNA - administration & dosage ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - immunology ; Viral Vaccines - administration & dosage</subject><ispartof>Journal of general virology, 2002-07, Vol.83 (7), p.1745-1751</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-ad1804f388625eaefef56eeb35506706e29071d6d436adb361024576c4e3eac03</citedby><cites>FETCH-LOGICAL-c379t-ad1804f388625eaefef56eeb35506706e29071d6d436adb361024576c4e3eac03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,3747,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12075094$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bharadwaj, Mausumi</creatorcontrib><creatorcontrib>Mirowsky, Katy</creatorcontrib><creatorcontrib>Ye, Chunyan</creatorcontrib><creatorcontrib>Botten, Jason</creatorcontrib><creatorcontrib>Masten, Barbara</creatorcontrib><creatorcontrib>Yee, Joyce</creatorcontrib><creatorcontrib>Lyons, C. Richard</creatorcontrib><creatorcontrib>Hjelle, Brian</creatorcontrib><title>Genetic vaccines protect against Sin Nombre hantavirus challenge in the deer mouse (Peromyscus maniculatus)</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Center for Emerging Infectious Diseases, Departments of Pathology 1 , Medicine 2 and Biology and Molecular Genetics and Microbiology 3 , University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
Author for correspondence: Brian Hjelle (at Department of Pathology). Fax +1 505 272 9912. e-mail bhjelle{at}salud.unm.edu
We used a deer mouse ( Peromyscus maniculatus ) infection model to test the protective efficacy of genetic vaccine candidates for Sin Nombre (SN) virus that were known to provoke immunological responses in BALB/c mice (Bharadwaj et al ., Vaccine 17, 28362843, 1999 ). Protective epitopes were localized in each of four overlapping cDNA fragments that encoded portions of the SN virus G1 glycoprotein antigen; the nucleocapsid gene also was protective. The protective efficacy of glycoprotein gene fragments correlated with splenocyte proliferation in the presence of cognate antigen, but none induced neutralizing antibodies. Genetic vaccines against SN virus can protect outbred deer mice from infection even in the absence of a neutralizing antibody response.</description><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>DNA, Complementary</subject><subject>Hantavirus Infections - immunology</subject><subject>Hantavirus Infections - prevention & control</subject><subject>Injections, Intramuscular</subject><subject>Mice</subject><subject>Nucleocapsid - genetics</subject><subject>Nucleocapsid - immunology</subject><subject>Peromyscus</subject><subject>Sin Nombre virus - genetics</subject><subject>Sin Nombre virus - immunology</subject><subject>Spleen - cytology</subject><subject>Spleen - immunology</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Viral Envelope Proteins - genetics</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Viral Vaccines - administration & dosage</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1P3DAQhi0EgmXpL0BCPiE4pLXjr-SIECxIqFQqPVuOM9kYEgdsh4p_j1e7gtMc5pl3Zh6ETin5SUld_yKkLAvKqCoqVqiCKi720IJyKYoy9_fR4os4QscxPhNCORfqEB3RkihBar5ALyvwkJzF78Za5yHi1zAlsAmbtXE-JvzXefx7GpsAuDc-mXcX5ohtb4YB_BpwbqcecAsQ8DjNEfDFHwjT-BFt5kbjnZ0Hk-Z4eYIOOjNE-LGrS_Tv9ubp-q54eFzdX189FJapOhWmpRXhHasqWQow0EEnJEDDhCBSEQllTRRtZcuZNG3DJCVlfktaDgyMJWyJzre5-ZW3GWLSo4sWhsF4yAdqRStOKioyyLagDVOMATr9GtxowoemRG8c641BvTGoK6aV3jjOU2e7-LkZof2e2UnNwMUW6N26_-8C6DX40eUljZt09ved9Qk3gIZv</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>Bharadwaj, Mausumi</creator><creator>Mirowsky, Katy</creator><creator>Ye, Chunyan</creator><creator>Botten, Jason</creator><creator>Masten, Barbara</creator><creator>Yee, Joyce</creator><creator>Lyons, C. Richard</creator><creator>Hjelle, Brian</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020701</creationdate><title>Genetic vaccines protect against Sin Nombre hantavirus challenge in the deer mouse (Peromyscus maniculatus)</title><author>Bharadwaj, Mausumi ; Mirowsky, Katy ; Ye, Chunyan ; Botten, Jason ; Masten, Barbara ; Yee, Joyce ; Lyons, C. Richard ; Hjelle, Brian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-ad1804f388625eaefef56eeb35506706e29071d6d436adb361024576c4e3eac03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>DNA, Complementary</topic><topic>Hantavirus Infections - immunology</topic><topic>Hantavirus Infections - prevention & control</topic><topic>Injections, Intramuscular</topic><topic>Mice</topic><topic>Nucleocapsid - genetics</topic><topic>Nucleocapsid - immunology</topic><topic>Peromyscus</topic><topic>Sin Nombre virus - genetics</topic><topic>Sin Nombre virus - immunology</topic><topic>Spleen - cytology</topic><topic>Spleen - immunology</topic><topic>Vaccines, DNA - administration & dosage</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Viral Vaccines - administration & dosage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bharadwaj, Mausumi</creatorcontrib><creatorcontrib>Mirowsky, Katy</creatorcontrib><creatorcontrib>Ye, Chunyan</creatorcontrib><creatorcontrib>Botten, Jason</creatorcontrib><creatorcontrib>Masten, Barbara</creatorcontrib><creatorcontrib>Yee, Joyce</creatorcontrib><creatorcontrib>Lyons, C. Richard</creatorcontrib><creatorcontrib>Hjelle, Brian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bharadwaj, Mausumi</au><au>Mirowsky, Katy</au><au>Ye, Chunyan</au><au>Botten, Jason</au><au>Masten, Barbara</au><au>Yee, Joyce</au><au>Lyons, C. Richard</au><au>Hjelle, Brian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic vaccines protect against Sin Nombre hantavirus challenge in the deer mouse (Peromyscus maniculatus)</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>2002-07-01</date><risdate>2002</risdate><volume>83</volume><issue>7</issue><spage>1745</spage><epage>1751</epage><pages>1745-1751</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>Center for Emerging Infectious Diseases, Departments of Pathology 1 , Medicine 2 and Biology and Molecular Genetics and Microbiology 3 , University of New Mexico School of Medicine, Albuquerque, NM 87131, USA
Author for correspondence: Brian Hjelle (at Department of Pathology). Fax +1 505 272 9912. e-mail bhjelle{at}salud.unm.edu
We used a deer mouse ( Peromyscus maniculatus ) infection model to test the protective efficacy of genetic vaccine candidates for Sin Nombre (SN) virus that were known to provoke immunological responses in BALB/c mice (Bharadwaj et al ., Vaccine 17, 28362843, 1999 ). Protective epitopes were localized in each of four overlapping cDNA fragments that encoded portions of the SN virus G1 glycoprotein antigen; the nucleocapsid gene also was protective. The protective efficacy of glycoprotein gene fragments correlated with splenocyte proliferation in the presence of cognate antigen, but none induced neutralizing antibodies. Genetic vaccines against SN virus can protect outbred deer mice from infection even in the absence of a neutralizing antibody response.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>12075094</pmid><doi>10.1099/0022-1317-83-7-1745</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1317 |
| ispartof | Journal of general virology, 2002-07, Vol.83 (7), p.1745-1751 |
| issn | 0022-1317 1465-2099 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71840815 |
| source | MEDLINE; Microbiology Society; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Animals Antibodies, Viral - blood DNA, Complementary Hantavirus Infections - immunology Hantavirus Infections - prevention & control Injections, Intramuscular Mice Nucleocapsid - genetics Nucleocapsid - immunology Peromyscus Sin Nombre virus - genetics Sin Nombre virus - immunology Spleen - cytology Spleen - immunology Vaccines, DNA - administration & dosage Viral Envelope Proteins - genetics Viral Envelope Proteins - immunology Viral Vaccines - administration & dosage |
| title | Genetic vaccines protect against Sin Nombre hantavirus challenge in the deer mouse (Peromyscus maniculatus) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T12%3A08%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20vaccines%20protect%20against%20Sin%20Nombre%20hantavirus%20challenge%20in%20the%20deer%20mouse%20(Peromyscus%20maniculatus)&rft.jtitle=Journal%20of%20general%20virology&rft.au=Bharadwaj,%20Mausumi&rft.date=2002-07-01&rft.volume=83&rft.issue=7&rft.spage=1745&rft.epage=1751&rft.pages=1745-1751&rft.issn=0022-1317&rft.eissn=1465-2099&rft_id=info:doi/10.1099/0022-1317-83-7-1745&rft_dat=%3Cproquest_cross%3E71840815%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=71840815&rft_id=info:pmid/12075094&rfr_iscdi=true |