Genetic vaccines protect against Sin Nombre hantavirus challenge in the deer mouse (Peromyscus maniculatus)

Center for Emerging Infectious Diseases, Departments of Pathology 1 , Medicine 2 and Biology and Molecular Genetics and Microbiology 3 , University of New Mexico School of Medicine, Albuquerque, NM 87131, USA Author for correspondence: Brian Hjelle (at Department of Pathology). Fax +1 505 272 9912....

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Veröffentlicht in:Journal of general virology 2002-07, Vol.83 (7), p.1745-1751
Hauptverfasser: Bharadwaj, Mausumi, Mirowsky, Katy, Ye, Chunyan, Botten, Jason, Masten, Barbara, Yee, Joyce, Lyons, C. Richard, Hjelle, Brian
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Sprache:eng
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Zusammenfassung:Center for Emerging Infectious Diseases, Departments of Pathology 1 , Medicine 2 and Biology and Molecular Genetics and Microbiology 3 , University of New Mexico School of Medicine, Albuquerque, NM 87131, USA Author for correspondence: Brian Hjelle (at Department of Pathology). Fax +1 505 272 9912. e-mail bhjelle{at}salud.unm.edu We used a deer mouse ( Peromyscus maniculatus ) infection model to test the protective efficacy of genetic vaccine candidates for Sin Nombre (SN) virus that were known to provoke immunological responses in BALB/c mice (Bharadwaj et al ., Vaccine 17, 2836–2843, 1999 ). Protective epitopes were localized in each of four overlapping cDNA fragments that encoded portions of the SN virus G1 glycoprotein antigen; the nucleocapsid gene also was protective. The protective efficacy of glycoprotein gene fragments correlated with splenocyte proliferation in the presence of cognate antigen, but none induced neutralizing antibodies. Genetic vaccines against SN virus can protect outbred deer mice from infection even in the absence of a neutralizing antibody response.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-83-7-1745